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Merck
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SCM304

Sigma-Aldrich

3dGRO® Human Colon Organoid Expansion Medium

Complete serum-free organoid media for the expansion and long-term 3D culture of human intestinal colon organoids.

别名:

Colon Organoid Culture Media, Intestinal Organoid Culture Media

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.71

形狀

liquid

包裝

pkg of 50 mL

技術

cell culture | stem cell: suitable

滲透度

350-375 mOsm

應用

cell analysis

一般說明

3dGRO® Human Colon Organoid Expansion Medium is a complete ready-to-use serum-free medium for the expansion and long-term culture of human colonic organoids. The medium has been validated for use with 3dGRO® Human iPSC Derived Colon Organoids (Cat. No. SCC300). Colon organoids propagated in the medium express colon-specific markers including the posterior hindgut marker CDX2, a-carbonic anydrase II (CA-II), a-carbonic anhydrase IV (CA-IV), and goblet cell markers Mucin-2 and Mucin-5B.

應用

Complete serum-free organoid media for the expansion and long-term 3D culture of human intestinal colon organoids.

Research Category:
  • Cell Culture
  • Stem Cell Research

品質

Appearance (Color): Clear/No Particlulates (Red Liquid)
Osmolality: 350-375 mOsm
pH: 7.0 – 7.4
Sterility Tested: No Growth/Pass
Endotoxin: <2 EU/mL
Mycoplasma: Negative
Functional Assay: Thaw and culture of human colon organoids for 2 passages.

儲存和穩定性

Upon receipt, store at −20°C. When ready to use, thaw overnight at 2-8°C. Once thawed, mix thoroughly. Use immediately and store at 2-8°C for up to 1 week. Do not re-freeze. Unused aliquots may be stored at −20°C until the expiry date.

法律資訊

3dGRO is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

WGK 1

閃點（°F）

Not applicable

閃點（°C）

Not applicable

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实验方案

Forskolin-Induced Swelling Assays

A rapid in vitro assay for CFTR function, the forskolin-induced swelling protocol uses human colon organoids, which can be derived from cystic fibrosis patient tissue.

In Vitro Differentiation of Human iPS Cells Into Colon Organoids in Serum-free Cell Culture Conditions

Highly characterized cryopreserved human colonic organoids and a step-by-step organoid culture protocol for epithelial intestinal organoid differentiation from iPS cells.

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