推荐产品
生物源
mouse
品質等級
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
PAb1620, monoclonal
形狀
liquid
包含
≤0.1% sodium azide as preservative
物種活性
human, mouse, rat, primate, bovine
製造商／商標名
Calbiochem®
儲存條件
do not freeze
同型
IgG2a
運輸包裝
wet ice
儲存溫度
2-8°C
目標翻譯後修改
unmodified
基因資訊
human ... TP53(7157)
一般說明
可识别Hs27细胞和乳腺癌组织中天然构象的~53 kDa野生型p53蛋白。不能识别突变或变性的p53蛋白。
该抗p53（Ab-5）（野生型）小鼠mAb（PAb1620）经验证可用于冰冻切片、免疫印迹、IF、IP和石蜡切片，以检测p53（Ab-5）（野生型）。
通过用指定的免疫原免疫BALB/c小鼠并将脾细胞与SP2/0 Ag14骨髓瘤细胞融合而产生的纯化小鼠单克隆抗体。可识别~53 kDa野生型p53蛋白。
免疫原
vLM 小鼠肿瘤细胞
應用
冰冻切片（见应用参考文献）
免疫印迹（不推荐）
免疫荧光（1-20 μg/ml）
免疫沉淀（1 μg/样品）
石蜡切片（5 μg/ml，需要加热预处理）
免疫印迹（不推荐）
免疫荧光（1-20 μg/ml）
免疫沉淀（1 μg/样品）
石蜡切片（5 μg/ml，需要加热预处理）
包裝
请参考特定浓度批号的标签。
警告
毒性：标准处理（A）
分析報告
阳性对照
Hs27细胞或乳腺癌组织
Hs27细胞或乳腺癌组织
其他說明
El-Deiry, W.S., et al. 1994.Cancer Res.54, 1169.
Greenblatt, M.S., et al. 1994.Cancer Res.54, 4855.
Barak, Y., et al. 1993.EMBO J.12, 461.
Kastan, M.B., et al. 1992.Cell71, 587.
Kuerbitz, S.J.1992.Proc.Natl.Acad.Sci. USA89, 7491.
Lane, D.P.1992.Nature358, 15.
Kastan, M.B., et al. 1991.Cancer Res.51, 6304.
Ball, R.K., et al. 1984.EMBO J.3, 1485.
Greenblatt, M.S., et al. 1994.Cancer Res.54, 4855.
Barak, Y., et al. 1993.EMBO J.12, 461.
Kastan, M.B., et al. 1992.Cell71, 587.
Kuerbitz, S.J.1992.Proc.Natl.Acad.Sci. USA89, 7491.
Lane, D.P.1992.Nature358, 15.
Kastan, M.B., et al. 1991.Cancer Res.51, 6304.
Ball, R.K., et al. 1984.EMBO J.3, 1485.
野生型p53的半衰期很短，并且在细胞中的含量很低。增加要免疫沉淀并施加到凝胶上的样品量可能有助于可视化。与35S-Met的短孵育时间（≤1小时）将有助于降低背景。p53（Ab-5）也可用于使用标准辣根过氧化物酶或免疫荧光检测技术观察细胞纺丝或培养细胞中的p53。p53（Ab-5）优先免疫沉淀野生型p53；它不应沉淀突变或变性的p53。在单个系统中，应对抗体进行滴定以获得最佳结果。
法律資訊
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
nwg
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Yasuhiro Hama et al.
Journal of pharmacological sciences, 110(4), 493-496 (2009-08-05)
We have previously demonstrated an important role of influx of Cl(-) rather than Ca(2+) in acute excitotoxicity in adult rat retina. As p53 has been implicated in delayed apoptotic cell death, here we examined the appearance of p53 immunoreactivity in
Maria L Gomez et al.
Oncogene, 38(29), 5751-5765 (2019-06-22)
We previously reported that the dismutase SOD1 is overexpressed in breast cancer. However, whether SOD1 plays an active role in tumor formation in vivo has never been demonstrated. Further, as luminal cells of normal breast epithelial cells are enriched in
Hyun-Lim Kim et al.
Molecules and cells, 38(4), 312-317 (2015-03-31)
Depletion of intracellular zinc by N,N,N',N'-tetrakis(2-pyridylmethyl) ethylenediamine (TPEN) induces p53-mediated protein synthesis-dependent apoptosis of mouse cortical neurons. Here, we examined the requirement for poly(ADP-ribose) polymerase (PARP)-1 as an upstream regulator of p53 in zinc depletion-induced neuronal apoptosis. First, we found
Alejandro Parrales et al.
Nature cell biology, 18(11), 1233-1243 (2016-10-28)
Stabilization of mutant p53 (mutp53) in tumours greatly contributes to malignant progression. However, little is known about the underlying mechanisms and therapeutic approaches to destabilize mutp53. Here, through high-throughput screening we identify statins, cholesterol-lowering drugs, as degradation inducers for conformational
Chinthalapally V Rao et al.
Neoplasia (New York, N.Y.), 15(9), 1018-1027 (2013-09-13)
Lung cancer is the leading cause of cancer deaths worldwide. Expression of the p53 tumor suppressor protein is frequently altered in tobacco-associated lung cancers. We studied chemopreventive effects of p53-modulating agents, namely, CP-31398 and Prima-1, on 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK)-induced lung adenoma
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