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Key Documents

OB05

Sigma-Aldrich

Anti-Histidine-Tagged Protein Mouse mAb (13/45/31/2)

liquid, clone 13/45/31/2, Calbiochem®

别名:

Anti-His6

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About This Item

分類程式碼代碼:
12352203
NACRES:
NA.41

生物源

mouse

品質等級

100

抗體表格

purified antibody

抗體產品種類

primary antibodies

無性繁殖

13/45/31/2, monoclonal

形狀

liquid

包含

≤0.1% sodium azide as preservative

物種活性（以同源性預測）

all

製造商／商標名

Calbiochem®

儲存條件

do not freeze

同型

IgG1

運輸包裝

wet ice

儲存溫度

2-8°C

目標翻譯後修改

unmodified

一般說明

Anti-Histidine-Tagged Protein, mouse monoclonal, clone 13/45/31/2, specifically recognizes an epitage of 6 consecutive His residues. It is validated for use in ELISA, WB, IF & IP.
Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with Sp2/0 mouse myeloma cells. Recognizes a His•Tag sequence in native and recombinant proteins. Specifically recognizes an epitope of six consecutive His residues of both natural and recombinant sources.
Specifically recognizes an epitage of six consecutive His residues of both natural and recombinant sources.

免疫原

Epitope: HHHHHH
a recombinant protein containing a His•Tag sequence

應用



ELISA (0.1-0.4 g/ml)
Immunoblotting (1-4 g/ml)
Immunofluorescence (1-4 g/ml)
Immunoprecipitation (4 g/ml)

包裝

Please refer to vial label for lot-specific concentration.

警告

Toxicity: Standard Handling (A)

外觀

In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.

分析報告

Positive Control
Proteins containing a His•Tag sequence

其他說明

Antibody should be titrated for optimal results in individual systems.
Zentgraf, H., et al. 1995. Nucl. Acids. Res.23, 3347.
Gu, J., et al. 1994. BioTechniques17, 257.
Sporeno, E., et al. 1994. J. Biol. Chem., 10991.
Sisk, W.P., et al. 1994. J. Virol.68, 766.
Lu, T., et al. 1993. Anal. Biochem.213, 318.
Garner, J., et al. 1992. Cell69, 833.
Hochuli, E., et al. 1987. J. Chromatogr.411, 177.

法律資訊

Manufactured by Dianova, GmbH, Germany.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 1

閃點（°F）

Not applicable

閃點（°C）

Not applicable

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Maureen C Dolan et al.
Methods in molecular biology (Clifton, N.J.), 824, 65-105 (2011-12-14)
Protein-specific antibodies serve as critical tools for detection, quantification, and characterization of recombinant proteins. Perhaps the most important and widely used antibody-based procedures for recombinant protein applications are Western immunoblotting and enzyme-linked immunosorbent assays (ELISAs). These analyses require well-characterized, sensitive
Jia Chen et al.
mBio, 10(1) (2019-02-21)
Kaposi's sarcoma-associated herpesvirus (KSHV) is a human gammaherpesvirus associated with the development of Kaposi's sarcoma (KS). KSHV target cells include endothelial cells, B cells, monocytes, epithelial cells, dendritic cells, macrophages, and fibroblasts. KSHV entry into target cells is a complex

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