推荐产品
生物源
mouse
品質等級
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
PC10, monoclonal
形狀
liquid
包含
≤0.1% sodium azide as preservative
物種活性
rat, yeast, mouse, human, zebrafish
製造商／商標名
Calbiochem®
儲存條件
do not freeze
同型
IgG2a
運輸包裝
wet ice
儲存溫度
2-8°C
目標翻譯後修改
unmodified
基因資訊
human ... PCNA(5111)
zebrafish ... Pcna(30678)
一般說明
识别HeLa细胞以及扁桃体或正常结肠组织中的~37 kDa PCNA蛋白。
该抗PCNA（Ab-1）小鼠mAb（PC10）经验证可用于免疫印迹、免疫荧光、免疫沉淀石蜡切片以检测PCNA（Ab-1）。
通过使用指定的免疫原免疫BALB/c小鼠并将脾细胞与Sp2/0-Ag14小鼠骨髓瘤细胞融合而产生的纯化的小鼠单克隆抗体。识别~37 kDa PCNA蛋白。
免疫原
表位：氨基酸112-121内
重组PCNA
應用
免疫印迹（2.5 µg/mL）
免疫荧光（1-5 µg/mL）
免疫沉淀（不建议使用未结合的抗体，请参见注释）
石蜡切片（2.5 µg/mL，无需预处理，请参见注释）
免疫荧光（1-5 µg/mL）
免疫沉淀（不建议使用未结合的抗体，请参见注释）
石蜡切片（2.5 µg/mL，无需预处理，请参见注释）
包裝
批次特定浓度请参见小瓶标签。
警告
毒性：标准处理（A）
分析報告
阳性对照
HeLa细胞、HL-60细胞、扁桃体组织或正常结肠组织
HeLa细胞、HL-60细胞、扁桃体组织或正常结肠组织
其他說明
Waseem, N.H. and Lane, D.P.1990.J. Cell.Sci.96, 121.
Suzuka, I., et al. 1989.Proc.Natl.Acad.Sci. USA86 3189.
Bravo, R., et al. 1987.Nature (London)326, 515.
Bravo, R. and MacDonald-Bravo, H. 1987.J. Cell.Biol.105, 1549.
Miyachi, K., et al. 1987.J. Immunol.121, 2228.
Suzuka, I., et al. 1989.Proc.Natl.Acad.Sci. USA86 3189.
Bravo, R., et al. 1987.Nature (London)326, 515.
Bravo, R. and MacDonald-Bravo, H. 1987.J. Cell.Biol.105, 1549.
Miyachi, K., et al. 1987.J. Immunol.121, 2228.
染色显示出强烈的核模式。不建议用于免疫沉淀，因为在某些样品中会沉淀出约37 kDa的蛋白质，而不是PCNA。通常，石蜡切片染色不需要预处理，但是在某些样品中，可以通过在37°C下用4 N HCl预处理10分钟，然后在水中洗涤3次来增强染色。在单个系统中，应对抗体进行滴定以获得最佳结果。
法律資訊
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
nwg
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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