跳转至内容
Merck
所有图片(3)

Key Documents

MABS835

Sigma-Aldrich

Anti-EGFR Antibody, clone 7F12.1

clone 7F12.1, from mouse

别名:

Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1

登录查看公司和协议定价


About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702

生物源

mouse

品質等級

抗體表格

purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

7F12.1, monoclonal

物種活性

human

技術

flow cytometry: suitable
immunocytochemistry: suitable
western blot: suitable

同型

IgG2bκ

NCBI登錄號

UniProt登錄號

運輸包裝

ambient

目標翻譯後修改

unmodified

基因資訊

human ... EGFR(1956)

一般說明

Epidermal growth factor receptor (EC 2.7.10.1; UniProt P00533; also known as Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1) is encoded by the EGFR (also known as ERBB, ERBB1, HER1, NISBD2) gene (Gene ID 1956) in human. The epidermal growth factor receptor (EGFR; Proto-oncogene c-ErbB-1) is a ubiquitously receptor tyrosine kinase that is activated by a variety of ligands. Upon ligand-bound EGFR undergoes dimerization and autophosphorylation exposing cytoplasmic domains, which allows its interaction with adaptor proteins and induction of several signaling pathways including PI3K-AKT; PLC-gamma-PKC; Notch1; and Ras-Raf. Hence, it contributes to a diverse range of cellular processes, including cell proliferation. Several studies have reported that EGFR signaling plays an important role in the development of various tumors. EGFR is regulated by PTPRJ and PTPRK phosphatases, and by the endocytic machinery including EPS15 that mediates internalization and degradation of the receptor.

特異性

Epitope region is present in spliced isoform 1 (p170), but not in isoform 2 (p60, Truncated, TEGFR), isoform 3 (p110) or isoform 4 reported by UniProt (P00533).

免疫原

GST-tagged recombinant human EGFR fragment correspoding to a cytoplasmic region C-terminal to the kinase domain.

應用

Detect EGFR using this mouse monoclonal , Cat. No. MABS835, validated for use in Flow Cytometry, Immunocytochemistry, and Western Blotting.
Flow Cytometry Analysis: 0.1 µg from a representative lot detected EGFR immunoreactivity in 4% paraformaldehyde-fixed, 0.2% Triton X-100-permeabilized A431 cells.

Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected plasma membrane EGFR immunoreactivity in 4% paraformaldehyde-fixed, 0.3% Triton X-100-permeabilized A431 cells.
Research Category
Signaling

品質

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: 0.1 µg/mL of this antibody detected EGFR in 10 µg of A431 cell lysate.

標靶描述

~170 kDa observed. Target band size appears larger than the calculated molecualar weight of 134.3 kDa due to glycosylation. Uncharacterized bands may be observed in some lysate(s).

聯結

Replaces: 04-338

外觀

Format: Purified
Purified mouse IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

未找到合适的产品?  

试试我们的产品选型工具.

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1


分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门