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Key Documents

MABS2054

Sigma-Aldrich

Anti-ABCG8 Antibody, clone 1B10A5

clone 1B10A5, from mouse

别名:

ATP-binding cassette sub-family G member 8, Sterolin-2

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.43

生物源

mouse

抗體表格

purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

1B10A5, monoclonal

物種活性

human, mouse, rat

包裝

antibody small pack of 25 μg

技術

immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable

同型

IgG2aκ

NCBI登錄號

UniProt登錄號

目標翻譯後修改

unmodified

基因資訊

mouse ... Abcg8(67470)

相关类别

一般說明

ATP-binding cassette sub-family G member 8 (UniProt: Q9DBM0, also known as Sterolin-2, ABCG8) is encoded by the Abcg8 gene (Gene ID: 67470) in murine species. ABCG8 is a multi-pass membrane protein that forms an obligate heterodimer with ABCG5 to mediate Mg2+ and ATP-dependent sterol transport across the cell membrane. It is required for normal sterol homeostasis and plays an essential role in the selective transport of the dietary cholesterol in and out of the enterocytes and in the selective sterol excretion by the liver into bile. ABCG8 and ABCG5 are primarily expressed in enterocytes and hepatocytes and their levels are up-regulated upon cholesterol feeding. They are shown to heterodimerize in the endoplasmic reticulum prior to being transported to apical membranes. Their ATPase catalytic domain is located N-terminal to the transmembrane domain. ABCG8 has an ABC transporter domain (aa 48-314) and a ABC transporter type-2 domain (aa 411-665). Two isoforms of ABCG8 have been described that are produced by alternative splicing. Inactivating mutations in either G5 or G8 are reported to cause sitosterolemia, a rare autosomal-recessive disorder characterized by hypercholesterolemia, phytosterolemia, and premature coronary artery disease. (Ref.: Graf, GA., et al. (2003). J. Biol. Chem. 278(48); 48275-48282.

特異性

Clone 1B10A5 detects ATP-binding cassette sub-family G member 8 (ABCG8). It targets an epitope with in the N-terminal half.

免疫原

Recombinant fragment corresponding to the first 350 amino acids from mouse ATP-binding cassette sub-family G member 8 (ABCG8).

應用

Anti-ABCG8, clone 1B10A5, Cat. No. MABS2054, is a mouse monoclonal antibody that detects ATP-binding cassette sub-family G member 8 and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
Immunocytochemistry Analysis: A representative lot detected ABCG8 in Immunocytochemistry applications (Wang, J., et. al. (2008). Biochemistry. 47(18):5194-204).

Immunoprecipitation Analysis: A representative lot detected ABCG8 in Immunoprecipitation applications (Graf, G.A., et. al. (2003). J Biol Chem. 278(48):48275-82).

Western Blotting Analysis: A representative lot detected ABCG8 in Western Blotting applications (Wang, J., et. al. (2008). Biochemistry. 47(18):5194-204; Graf, G.A., et. al. (2003). J Biol Chem. 278(48):48275-82).
Research Category
Signaling

品質

Evaluated by Immunohistochemistry (Paraffin) in mouse colon tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected ABCG8 in mouse colon tissue sections.

標靶描述

75.99 kDa calculated.

外觀

Protein G purified
Format: Purified
Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Jin Wang et al.
Biochemistry, 47(18), 5194-5204 (2008-04-12)
ABCG5 (G5) and ABCG8 (G8) are ATP-binding cassette half-transporters that limit intestinal uptake and promote biliary secretion of neutral sterols. Here, we describe the purification of endogenous G5G8 from mouse liver to near homogeneity. We incorporated the native proteins into
Gregory A Graf et al.
The Journal of biological chemistry, 278(48), 48275-48282 (2003-09-25)
ABCG5 (G5) and ABCG8 (G8) are ATP-binding cassette (ABC) transporters that limit intestinal absorption and promote biliary excretion of neutral sterols. Mutations in either ABCG5 or ABCG8 result in an identical clinical phenotype, suggesting that these two half-transporters function as

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