MABN2434M

Anti-Retinoschisin Antibody, clone 3R10

clone 3R10, from mouse

• 别名: X-linked juvenile retinoschisis protein, RS1-3R10
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702

属性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: 3R10, monoclonal
• 物種活性: human, mouse
• 包裝: antibody small pack of 25 μg
• 技術: immunofluorescence: suitable; western blot: suitable
• 同型: IgG2aκ
• NCBI登錄號: NP_000321
• UniProt登錄號: O15537
• 目標翻譯後修改: unmodified
• 基因資訊: human ... RS1(6247)

说明

一般說明

Retinoschisin (UniProt: O15537; also known as X-linked juvenile retinoschisis protein) is encoded by the RS1 (also known as XLRS1) gene (Gene ID: 6247) in human. Retinoschisin is discoidin domain-containing protein that is localized along the extracellular surfaces of rod and cone photoreceptors and bipolar cells including the synapses. It is undetectable in the inner plexiform layers and the inner nuclear layer. It is active in cell adhesion process during retinal development and maintains the cellular organization and synaptic structure of the retina. Retinoschisin is a homooctamer of 4 homodimers that are linked by disulfide bonds. It contains a single F5/8 type C domain. Deleterious mutations in RS1 encoding retinoschisin are associated with X-linked juvenile retinoschisis (RS), a common form of macular degeneration in males, which is characterized by a negative electroretinogram pattern and by a splitting of the inner retina. Approximately half of cases of X-linked retinoschisis have bilateral peripheral retinoschisis in the inferotemporal part of the retina. Retinoschisin levels are shown to be up-regulated during the differentiation of a retinoblastoma cell line.

特異性

Clone 3R10 detects Retinoschisin in murine retinal cells. It targets an epitope within 18 amino acids from the N-terminal region.

免疫原

GST-tagged fusion protein containing a the LSSTEDEGEDPWYQKAC peptide fragment corresponding to amino acids 22-39 from the N-terminal region of human Retinoschisin precusor protein.

應用

Anti-Retinoschisin, clone 3R10, Cat. No. MABN2434, is a highly specific mouse monoclonal antibody that targets Retinoschisin and has been tested in Immunofluorescence and Western Blotting..

Immunofluorescence Analysis: A representative lot detected Retinoschisin in Immunofluorescence applications (Min, S.H., et. al. (2005). Mol Ther. 12(4):644-51; Weber, B.H., et. al. (2002). Proc Natl Acad Sci USA. 99(9):6222-7).

Western Blotting Analysis: A representative lot detected Retinoschisin in Western Blotting applications (Min, S.H., et. al. (2005). Mol Ther. 12(4):644-51; Wu, W.W., et. al. (2003). J Biol Chem. 278(30):28139-46).

Research Category
Neuroscience

品質

Evaluated by Western Blotting in mouse retinal tissue lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected Retinoschisin in 10 µg of mouse retinal tissue lysate.

標靶描述

~23 kDa observed; 25.59 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外觀

Protein G purified

Format: Purified

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable