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生物源
mouse
品質等級
共軛
unconjugated
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
3A7.10, monoclonal
分子量
calculated mol wt 23.72 kDa
observed mol wt ~21 kDa
純化經由
using protein G
物種活性
human
包裝
antibody small pack of 100 μg
技術
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
同型
IgG1κ
表位序列
Unknown
Protein ID登錄號
UniProt登錄號
運輸包裝
ambient
目標翻譯後修改
unmodified
基因資訊
mouse ... IL6(3569)
一般說明
Interleukin-6 (UniProt: P05231; also known as IL-6, B-cell stimulatory factor 2, BSF-2, CTL differentiation factor, CDF, Hybridoma growth factor, Interferon beta-2, IFN-beta-2) is encoded by the IL6 (also known as IFNB2) gene (Gene ID: 3569) in human. IL-6 is an immunoregulatory secreted cytokine that is synthesized with a signal peptide (aa 1-29), which is subsequently cleaved off to produce the mature form (aa 30-212). It is mainly produced in skeletal muscle and its levels are shown to increase upon exercise due to enhanced production by contracting skeletal muscles. It is a potent inducer of the acute phase response and rapid IL-6 production contributes to host defense during infection and tissue injury. In the innate immune response, it is synthesized by myeloid cells, such as macrophages and dendritic cells, upon recognition of pathogens through toll-like receptors (TLRs) at the site of infection. In the adaptive immune response, it is required for the differentiation of B cells into immunoglobulin-secreting cells. It is reported to plays a major role in the differentiation of CD4+ T cell subsets and is an essential factor for the development of T follicular helper (Tfh) cells. Its actions are mediated by binding to IL-6 receptor (IL-6R) and the ligand-receptor complex associates to the signaling subunit IL6ST/gp130. The interaction with the membrane-bound IL6R and IL6ST is reported to stimulate the classic signaling, whereas the binding of IL6 and soluble IL6R to IL6ST stimulates trans-signaling where synergistically with IL-1b and TNF it can induce the production of VEGF and stimulate angiogenesis. (Ref.: Nakahara, H., et al. (2003). Arthritis Rheum. 48(6); 1521-1529; Boulanger, MJ., et al. (2003). Science. 300(5628); 2101-2104).
特異性
Clone 3A7.10 is a mouse monoclonal antibody that detects human IL-6.
免疫原
Recombinant fragment of human Interleukin 6 (IL-6) corresponding to amino acids 29-212.
應用
Quality Control Testing
Evaluated by Western Blotting with recombinant human IL-6.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected recombinant human IL-6.
Tested Applications
Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected IL-6 in human tonsil tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting with recombinant human IL-6.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected recombinant human IL-6.
Tested Applications
Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected IL-6 in human tonsil tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-IL-6, clone 3A7.10, Cat. No. MABF3179, is a mouse monoclonal antibody that detects human Interleukin-6 and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
外觀
Purified mouse monoclonal antibody IgG1 in PBS with 0.05% sodium azide.
儲存和穩定性
Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類(WGK)
WGK 2
閃點(°F)
Not applicable
閃點(°C)
Not applicable
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