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Merck
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Key Documents

MABE462

Sigma-Aldrich

抗-TET2抗体,克隆hT2H 21F11

clone hT2H21F11, from mouse

别名:

Methylcytosine dioxygenase TET2

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

mouse

品質等級

抗體表格

purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

hT2H21F11, monoclonal

物種活性

human, mouse

技術

ChIP: suitable
immunoprecipitation (IP): suitable
western blot: suitable

同型

IgG1κ

NCBI登錄號

UniProt登錄號

運輸包裝

wet ice

目標翻譯後修改

unmodified

基因資訊

human ... TET2(54790)

一般說明

甲基胞嘧啶双加氧酶TET2催化甲基胞嘧啶(5mC)向5-羟甲基胞嘧啶(5hmC)的转化。目前尚不清楚5-羟甲基胞嘧啶的功能,但它可能影响染色质结构,或充当胞嘧啶去甲基化的中间成分。TET2经常在骨髓增生性疾病(MPD)或骨髓增生性肿瘤(MPN)和全身性肥大细胞增多症中发生突变。TET2疾病还会引起真性红细胞增多症(PV)和骨髓增生异常综合症(MDS)。

特異性

该抗体识别TET2的N末端

免疫原

对应于人TET2 N末端的重组蛋白。

應用

使用该小鼠单克隆抗体(抗TET2抗体,克隆hT2H 21F11)检测Tet2,该抗体经验证可用于染色质IP(ChIP)和蛋白质印迹& IP。
蛋白质印迹分析:1.0 µg/mL的代表性批次在10 µg TF-1细胞裂解物中检测到TET2。
免疫沉淀分析:5 µg的代表性批次在250 µg HL60 RIPA细胞裂解物中免疫沉淀TET2。

品質

通过蛋白质印迹法在MOLT-4细胞裂解物中进行评价。

蛋白质印迹分析:1.0 µg/mL的该抗体在10µg MOLT-4细胞裂解物中检测到TET2。

標靶描述

观测分子量〜260 kDa。该蛋白的计算分子量为224 kDa,但可在约260 kDa处观察到

外觀

形式:纯化

分析報告

对照
MOLT-4细胞裂解物

其他說明

浓度:关于批次特定浓度请参见检验报告。

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Lorenzo de la Rica et al.
Genome biology, 14(9), R99-R99 (2013-09-14)
DNA methylation is a key epigenetic mechanism for driving and stabilizing cell-fate decisions. Local deposition and removal of DNA methylation are tightly coupled with transcription factor binding, although the relationship varies with the specific differentiation process. Conversion of monocytes to
Yueyue Duan et al.
Journal of medical virology, 94(7), 3251-3256 (2022-02-26)
Swine acute diarrhea syndrome coronavirus (SADS-CoV) is a newly discovered bat-origin coronavirus with fatal pathogenicity for neonatal piglets. There is no vaccine to prevent SADS-CoV infection or clinically approved drugs targeting SADS-CoV. Therefore, unraveling cellular factors that regulate SADS-CoV for
Yan-Ping Xu et al.
The Journal of clinical investigation, 130, 4316-4331 (2019-07-17)
Loss-of-function mutations in genes encoding TET DNA dioxygenase occur frequently in hematopoietic malignancy, but rarely in solid tumors which instead commonly have reduced activity. The impact of decreased TET activity in solid tumors is not known. Here we show that
Yundong He et al.
Nature communications, 12(1), 1521-1521 (2021-03-23)
Resistance to next-generation anti-androgen enzalutamide (ENZ) constitutes a major challenge for the treatment of castration-resistant prostate cancer (CRPC). By performing genome-wide ChIP-seq profiling in ENZ-resistant CRPC cells we identify a set of androgen receptor (AR) binding sites with increased AR
Eevi Kaasinen et al.
Nature communications, 10(1), 1252-1252 (2019-03-21)
Clonal hematopoiesis driven by somatic heterozygous TET2 loss is linked to malignant degeneration via consequent aberrant DNA methylation, and possibly to cardiovascular disease via increased cytokine and chemokine expression as reported in mice. Here, we discover a germline TET2 mutation

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