推荐产品
生物源
mouse
品質等級
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
528, monoclonal
形狀
liquid
包含
≤0.1% sodium azide as preservative
物種活性
human
應無反應活性
mouse, rat
製造商／商標名
Calbiochem®
儲存條件
do not freeze
同型
IgG2a
運輸包裝
wet ice
儲存溫度
2-8°C
目標翻譯後修改
unmodified
基因資訊
human ... EGFR(1956)
mouse ... Egfr(13649)
rat ... Egf(25313)
一般說明
Anti-EGFR (Ab-1), mouse monoclonal, clone 528, recognizes EGF receptor protein in A431 cells and normal skin. It is validated for use in ELISA, IF, IP, IHC (frozen,paraffin) & neutralization studies.
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references. Recognizes the ~170 kDa EGF receptor.
Recognizes the ~170 kDa EGF receptor protein in A431 cells and normal skin.
應用
Flow Cytometry (5 µg/ml)
Frozen Sections (2.5 µg/ml)
Immunofluorescence (5 µg/ml)
Immunoprecipitation(1 µg/sample)
Neutralization Studies (see comments and application references)
Paraffin Sections (see application references)
Frozen Sections (2.5 µg/ml)
Immunofluorescence (5 µg/ml)
Immunoprecipitation(1 µg/sample)
Neutralization Studies (see comments and application references)
Paraffin Sections (see application references)
包裝
Please refer to vial label for lot-specific concentration.
警告
Toxicity: Standard Handling (A)
分析報告
Negative Control
HL-60 cells or human brain
HL-60 cells or human brain
Positive Control
A-431 or normal skin tissue
A-431 or normal skin tissue
其他說明
Hunter T. 1984. Nature311, 414.
Masui, H., et al. 1984. Cancer Res.44, 1002.
Reynolds, F. H., Jr., et al. 1981. Nature 292, 259.
Ushiro, H. and Cohen, S. 1980. J. Biol. Chem.255, 8363.
Masui, H., et al. 1984. Cancer Res.44, 1002.
Reynolds, F. H., Jr., et al. 1981. Nature 292, 259.
Ushiro, H. and Cohen, S. 1980. J. Biol. Chem.255, 8363.
This antibody inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity (see application references). Also inhibits A431 tumor formation in nude mice. For neutralization studies, use Cat. No. GR01L; Cat. No. GR01 contains azide, which is toxic to cells. Antibody should be titrated for optimal results in individual systems.
法律資訊
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
nwg
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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