生物源
mouse
抗體表格
purified from hybridoma cell culture
抗體產品種類
primary antibodies
無性繁殖
29.1, monoclonal
形狀
buffered aqueous solution
分子量
~170 kDa
物種活性
human, bovine, mouse
包裝
antibody small pack of 25 μL
濃度
~1 mg/mL
技術
immunoblotting: 0.5-1 μg/mL using human epidermoid carcinoma A431 cell line extract.
immunofluorescence: 2-4 μg/mL using human epidermoid carcinoma A431 cells.
同型
IgG1
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
基因資訊
human ... EGFR(1956)
一般說明
Epidermal growth factor receptor (EGFR), also known as Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1 or HER-1, is an integral cell membrane protein which spans the membranes of a wide range of normal and malignant epithelial cells. EGFR is a tyrosine-specific protein kinase domain, it has an extracellular region which binds EGF and an intra-cellular carboxy-terminal region which possesses the tyrosine kinase activity. EGF binding to its specific EGFR, results in an increased DNA synthesis initiating a signaling cascades including cell proliferation, differentiation and repair of damaged epithelial tissue.
免疫原
Paraformaldehyde-fixed carcinoma cell line A431
應用
Monoclonal Anti-Epidermal Growth Factor (EGF) Receptor specifically bind to a carbohydrate residue on the external region of the EGF receptor molecule with no interference to the binding of EGF to its receptor, nor does it influence the binding of other monoclonal antibodies to the EGF Receptor. Reactivity has been observed with EGF receptors from mouse, human and bovine origin. The antibody is recommended to use in various immunological techniques, including Immunobloting (~170 kDa), Immunoprecipitation, flow cytometry (FACS), Immunohistochemistry and Immunofluorescence.
外觀
Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.
其他說明
This product is for R&D use only, not for drug, household, or other uses.
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
E Gerdin et al.
American journal of clinical pathology, 99(1), 28-31 (1993-01-01)
It has been reported that endothelium in malignant glioma stains with a commercial antibody raised against the receptor for epidermal growth factor (EGFr) on A431 cells (clone 29.1). In this report, this antibody was used to study the immunohistochemical expression
Y A Kita et al.
FEBS letters, 349(1), 139-143 (1994-07-25)
Her3/erbB3 has been identified as a third member of the epidermal growth factor receptor (EGFR) family [(1989) Proc. Natl. Acad. Sci. USA 86, 9193-9197; (1990) Proc. Natl. Acad. Sci. USA 87, 4905-4909]. The natural ligand for Her3 has not been
L H Defize et al.
The Journal of cell biology, 107(3), 939-949 (1988-09-01)
The epidermal growth factor receptor (EGF-R) of human A431 cells bears an antigenic determinant that is closely related to the human blood group A carbohydrate structure. Labeling studies with blood group A reactive anti-EGF-R monoclonal antibodies and various lectins revealed
Y Yarden et al.
The Journal of biological chemistry, 260(1), 315-319 (1985-01-10)
A method is described for a rapid two-step purification of the membrane receptor for epidermal growth factor (EGF) from cultured human A-431 cells. After solubilization of the cells with Triton X-100, the receptor is immobilized on an immunoaffinity column containing
Quantitative analysis of endocytosis and turnover of epidermal growth factor (EGF) and EGF receptor.
Alexander Sorkin et al.
Current protocols in cell biology, Chapter 15, Unit 15-Unit 15 (2010-03-18)
Binding of epidermal growth factor (EGF) to the EGF receptor (EGFR) initiates signal transduction, ultimately leading to altered gene expression. Ligand-activated EGFR is also rapidly internalized and then targeted to lysosomes for degradation or recycled back to the plasma membrane.
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