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FC010

Sigma-Aldrich

人血浆纤连蛋白纯化蛋白

This Human plasma fibronectin is a purified protein, used as an attachment factor suitable for cell propagation in vitro.

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About This Item

分類程式碼代碼:
12352202
eCl@ss:
32160405
NACRES:
NA.75

生物源

human

品質等級

100
300

無菌

sterile; sterile-filtered

化驗

~95% (SDS-PAGE)

形狀

liquid

分子量

220 kDa

製造商／商標名

Chemicon®

濃度

1 mg/mL

技術

cell culture | mammalian: suitable

表面覆盖率

2—10 μg/cm2

輸入

sample type mesenchymal stem cell(s)
sample type neural stem cell(s)
sample type: human embryonic stem cell(s)
sample type epithelial cells
sample type hematopoietic stem cell(s)
sample type induced pluripotent stem cell(s)
sample type pancreatic stem cell(s)

NCBI登錄號

NM_002026.2
NM_054034.2
NM_212474.1
NM_212475.1
NM_212476.1
NM_212478.1
NM_212482.1

UniProt登錄號

P02751

結合特異性

Peptide Source: Collagen

Peptide Source: Laminin

運輸包裝

wet ice

儲存溫度

2-8°C

基因資訊

human ... FN1(2335)

相关类别

一般說明

纤连蛋白（FN）是细胞外基质（ECM）和体液中的高分子量粘着糖蛋白。它是由两个二硫键连接的两个亚基组成的二聚体。FN的每个单体由三个重复单元组成：12个I 型FN重复单元（每个单元含40个左右氨基酸）、两个II型重复单元（大约60个氨基酸）和15-17个III型重复单元（大约90个氨基酸）。这些重复单元有助于形成纤连蛋白分子结构和功能的多样性。

應用

人纤连蛋白（HFN）当用于涂覆细胞培养表面（包括塑料制品、玻璃器皿和微载体珠子）时，适合用作体外细胞繁殖的附着因子。

生化／生理作用

纤连蛋白在各种细胞相互作用的介导中起关键作用，并参与各种过程，包括细胞粘附、细胞形态的建立和维持、细胞迁移、生长和分化。它与多种细胞外基质（ECM）和细胞表面蛋白（如胶原蛋白）、肝素、血纤维蛋白和特定细胞膜受体相互作用。纤连蛋白也可作为多种整联蛋白的的配体，包括总所周知的纤连蛋白受体 α5β1，该受体结合纤连蛋白III-10 重复区域中的精氨酰-甘氨酰-天冬氨酸序列。这种广泛的相互作用网络突显了纤连蛋白在调节细胞行为和介导组织功能中的重要性。

外觀

液体50 mM Tris缓冲盐溶液(TBS)， pH 7.5，无菌过滤，不含防腐剂。

儲存和穩定性

自收到之日起，在2-8°C可最多保存6个月。 请勿冷冻。

分析報告

220 kDa 的双带在还原条件下存在。hFN 是通过在明胶琼脂糖上的亲和层析，然后在肝素-琼脂糖上层析进行纯化的。已筛选出来自供体的血浆，并显示对 HIV、HTLV、乙型肝炎和丙型肝炎呈阴性。

其他說明

涂覆细胞培养皿的建议操作步骤

1.将总表面积（cm2）乘以目标HFN 浓度（μg/mL），确定覆盖培养皿所需的 HFN 量。建议用量为 2-10 μg/cm2.。

2.采用可涂覆整个培养皿的最少量的无菌平衡盐溶液（无血清和蛋白），润湿每个培养皿的表面。

3.如果需要，通入合适的CO2气氛。

4.将计算出的 HFN 量添加到每个培养皿中。

5.让 HFN 吸附到容器表面 5-20 分钟。

6.在进行标准细胞培养步骤之前去除残留的平衡盐溶液

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

仅供研究使用在法国，在用于科研目的时（包括进口和出口活动（《公共健康法》第L 1211-1条，第2节））该产品为管制品。购买者（即最终用户）需要获得《公共健康法》法第L 1211-1条规定的法国研究部的进口授权。订购本产品即表明您确认已经获得合适的进口授权。
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

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Intestinal epithelial wound healing assay in an epithelial-mesenchymal co-culture system.
Seltana, Amira, et al.
Wound Repair and Regeneration, 18, 114-122 null
Johannes Rheinlaender et al.
Nature materials, 19(9), 1019-1025 (2020-05-27)
Cortical stiffness is an important cellular property that changes during migration, adhesion and growth. Previous atomic force microscopy (AFM) indentation measurements of cells cultured on deformable substrates have suggested that cells adapt their stiffness to that of their surroundings. Here
Aleksander A Rebane et al.
FEBS letters, 594(7), 1132-1144 (2019-12-14)
Golgins are an abundant class of peripheral membrane proteins of the Golgi. These very long (50-400 nm) rod-like proteins initially capture cognate transport vesicles, thus enabling subsequent SNARE-mediated membrane fusion. Here, we explore the hypothesis that in addition to serving as
Mattia Usuelli et al.
The Journal of biological chemistry, 296, 100584-100584 (2021-03-28)
Physical interactions between vascular endothelial growth factor (VEGF), a central player in blood endothelial cell biology, and fibronectin, a major fibrillar protein of the extracellular matrix, are important determinants of angiogenic activity in health and disease. Conditions signaling the need
E Ruoslahti et al.
The Biochemical journal, 193(1), 295-299 (1981-01-01)
Human fibronectin was isolated from second-trimester amniotic fluid, from amniotic fluid obtained at term and from adult plasma. The amniotic-fluid fibronectins had a slightly higher apparent molecular weight on sodium dodecyl sulphate/polyacrylamide-gel electrophoresis than the plasma fibronectin. Early- and late-amniotic-fluid
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