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Merck
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文件

10838039001

Roche

纤连蛋白

from human plasma

别名:

纤连蛋白 溶液 来源于人类成纤维细胞

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About This Item

MDL號碼:
MFCD00131062
分類程式碼代碼:
12352207

生物源

human plasma

品質等級

100

無菌

non-sterile; 0.2 μm filtered

化驗

95% (SDS-PAGE)

形狀

lyophilized (clear, colorless solution after reconstitution)

分子量

440 kDa

包裝

pkg of 5 mg

製造商／商標名

Roche

儲存條件

avoid repeated freeze/thaw cycles

技術

cell culture | mammalian: suitable

UniProt登錄號

P02751

儲存溫度

2-8°C

基因資訊

human ... FN1(2335)

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相关类别

一般說明

纤连蛋白负责细胞与细胞外基质的相互作用。参与细胞的黏附、迁移、生长和分化。是血浆和其他体液的可溶性成分。此外，它也是不溶性细胞外基质的一部分。

特異性

对大多数哺乳动物细胞有活性

應用

纤维连接蛋白促进许多细胞的附着和随后的扩散。用于培养皿的包被。

測試方式

促进 3T3 (NR6) 细胞粘附的检测（XTT 切割）

外觀

在冻干过滤通过 0.2μm 孔径的膜之前，冷冻干燥物（每 mg 纤连蛋白含有 1.126mg 甘氨酸和 0.058mg 氯化钠）。

準備報告

工作浓度： 5μg/cm 2
5μg/cm 2 对于细胞培养容器的包衣
储存条件（工作液）：-15°C 至-25°C
复溶溶液在-15°C 至-25°C 下稳定。
注意： 将复溶溶液分等份储存于-15 至-25°C 条件下。
避免反复冻融。

重構

分别加入 1 或 5 mL 无菌水，得到最终浓度为 1 mg/mL 的溶液。在 37°C 下孵育 30 至 60 min 以溶解。 不要搅拌。
 复溶后，溶液可能含有少量不溶性聚集物。这些聚集体是纤连蛋白固有的现象，不影响产品性能。

其他說明

仅用于生命科学研究。不可用于诊断。

免責聲明

仅供研究使用。在法国，在用于科研目的时（包括进口和出口活动（《公共健康法》第L 1211-1条，第2节））该产品为管制品。购买者（即最终用户）需要获得《公共健康法》法第L 1211-1条规定的法国研究部的进口授权。订购本产品即表明您确认已经获得合适的进口授权。

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 1

閃點（°F）

does not flash

閃點（°C）

does not flash

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Daniella Penko et al.
Islets, 3(3), 73-79 (2011-04-12)
Pancreatic islet transplantation is limited by extensive apoptosis and suboptimal function of the implanted islets in the longer term. Endothelial progenitor cells (EPC) may be ideal for enhancing both the survival and function of transplanted islets. Here, we describe for
Daniella Penko et al.
Cell transplantation, 24(1), 37-48 (2013-09-28)
The success of pancreatic islet transplantation is limited by delayed engraftment and suboptimal function in the longer term. Endothelial progenitor cells (EPCs) represent a potential cellular therapy that may improve the engraftment of transplanted pancreatic islets. In addition, EPCs may
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A missense change in RRAS2 (Gln72 to Leu), analogous to the Gln61-to-Leu mutation of RAS oncoproteins, has been identified as a long-tail hotspot mutation in cancer and Noonan syndrome. However, the relevance of this mutation for in vivo tumorigenesis remains understudied.
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An orchestrated wound healing program drives skin repair via collective epidermal cell proliferation and migration. However, the molecular determinants of the tissue microenvironment supporting wound healing remain poorly understood. Herein we discover that proteoglycan Agrin is enriched within the early
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The eggs of the parasitic blood fluke, Schistosoma, are the main drivers of the chronic pathologies associated with schistosomiasis, a disease of poverty afflicting approximately 220 million people worldwide. Eggs laid by Schistosoma mansoni in the bloodstream of the host
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