AP202P

Goat Anti-Rat light chain Antibody, HRP conjugate

Chemicon^®, from goat

• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

属性

• 生物源: goat
• 品質等級: 100
• 共軛: peroxidase conjugate
• 抗體表格: affinity purified immunoglobulin
• 抗體產品種類: secondary antibodies
• 無性繁殖: polyclonal
• 物種活性: rat
• 製造商／商標名: Chemicon^®
• 技術: ELISA: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• 同型: IgG
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified

说明

一般說明

Light chains covalently bound to a pair of heavy chains to form an antibody molecule. Immunoglobulin light chains occur in two types, kappa or lambda. Light chain′s mass is about 25,000 Daltons per mole. Each light chain consists of about 250 amino-acids long. These are usually designated by the Greek letters kappa and lambda. The ratio of kappa to lambda found in the immunoglobulin population varies by species.

特異性

Minimal cross-reaction with bovine, goat, horse, mouse, human, rabbit, and sheep.

The antibody reacts strongly with native primary antibodies primarily with kappa light chains. It is not suitable for detecting lambda light chains. The antibody does not react with the heavy chain of rat IgG. The antibody has been tested by ELISA and adsorbed to ensure minimal cross-reaction with bovine, goat, horse, mouse, human, rabbit, and sheep immunoglobulins.

免疫原

Epitope: Kappa light chain

Prepared from purified rat IgG light chain.

應用

Goat anti-Rat light chain Antibody, HRP conjugate is an antibody against light chain for use in ELISA, WB, IC, IH.

Research Category
Secondary & Control Antibodies

Research Sub Category
Fragment Specific Secondary Antibodies

Western Blotting: 1:5,000 -1:100,000 with chromogenic substrates
1:10,000 -1:200,000 with ECL substrates
ELISA: 1:5,000 -1:100,000
Immunohistochemistry: 1:500 - 1:5,000
Immunocytochemistry: 1:500 - 1:5,000
Optimal working dilutions must be determined by the end user.

標靶描述

25 kDa

外觀

ImmunoAffinity Purified

Purified by immunoaffinity chromatography. Lyophilized in 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6. 15 mg/mL BSA as stabilizer.

儲存和穩定性

Maintain lyophilized product at 2°-8°C for up to 12 months. Reconstitute vial with of distilled water (addition of 0.5 ml water will yield a final concentration of 1 mg/mL). After reconstitution the product is stable for six weeks at 2°-8°C in the dark as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze thaw cycles. Keep away from light.

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3