ABT95

Anti-TGN46, Antibody

from rabbit, purified by affinity chromatography

• 别名: Trans-Golgi network integral membrane protein 2, TGN38 homolog, TGN46, TGN48, Trans-Golgi network protein TGN51
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

属性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 純化經由: affinity chromatography
• 物種活性: mouse, rat, human
• 物種活性（以同源性預測）: canine (based on 100% sequence homology)
• 技術: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• NCBI登錄號: NP_006455
• UniProt登錄號: O43493
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... TGOLN2(10618)

说明

一般說明

TGN46 is one of several proteins that utilize retrograde transport to travel between the trans-Golgi network (TGN) and the endocytic pathway. Retrograde transport involves the tethering, docking, fusion, and post translational modification of TGN proteins. Further studies are still required to elucidate the roles and mode of action for TGN46.

特異性

This antibody recognizes the cytoplasmic domain of TGN46.

免疫原

Epitope: Cytoplasmic domain

KLH-conjugated linear peptide corresponding to the cytoplasmic domain of human TGN46.

應用

Anti-TGN46 detects levels of TGN46 proteins & has been published & validated for use in wb, IP & ICC.

Research Category
Cell Structure

Research Sub Category
Vesicular Trafficking

Western Blot Analysis: 2 µg/mL of this antibody detected TGN46 in 10 µg of NIH/3T3 cell lysate. Multiple uncharacterized bands may be observed in some cell lysates.

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected TGN46 in A431 and HeLa cells.

品質

Evaluated by Immunoprecipitation in HeLa cell lysate.

Immunoprecipitation Analysis: 0.2 µg of this antibody immunoprecipitated TGN46 in 500 µg of HeLa cell lysate (An uncharacterized band at ~29 kDa may be observed in some cell lysates.).

標靶描述

~110 kDa observed. This protein is observed at ~110 kDa, and it is subject to glycosylation (Khanobdee, K. et al. (2004). Mol Vis. 13(10):933-42.; Prescott, A. R., et al. (1997). Eur J Cell Biol. 72(3):238-246.).

外觀

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

分析報告

Control
HeLa cell lysate

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable