ABN1672

Anti-HD-polyAla Ct

serum, from rabbit

• 别名: HD-RAN-PolyAla, HD-PolyAla
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702

属性

• 生物源: rabbit
• 抗體表格: serum
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 物種活性: mouse, human
• 包裝: antibody small pack of 25 μL
• 技術: immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• 同型: IgG
• 目標翻譯後修改: unmodified

说明

一般說明

Huntington disease (HD) is a progressive neurodegenerative disorder is caused by a CAG,CTG expansion in the HTT gene that results in the production of a mutant huntingtin protein (HTT) with polymeric expansions that accumulate in human brain with HD. PolyAla is a sense repeat-associated non-ATG (RAN) translation protein that accumulates in various brain regions with some toxic effects leading to microglial activation and neuronal loss. HD-RAN polyAla toxicity is shown to be lower compared to that of polyGln. The transfection of cells with polyAla construct can express high polyAla levels, but have lower toxicity. HD-polyAla RAN proteins are reported to accumulate in cells expressing CAG repeats that are equal to or greater than 52 but not at repeats less than 45. HD-RAN proteins are abundant in regions of the brain that are most affected by HD and these regions display pathologic features of HD, such as caspase-3 activity and microglial activation. HD-Ran protein accumulation and aggregation in HD brains are shown to be length dependent.

特異性

This rabbit polyclonal antibody specifically detects RAN proteins with PolyAla.

免疫原

A synthetic peptides from the C-terminal regions of the predicted 21 polyAla frame of HD in the CAG direction.

應用

Anti-HD-polyAla Ct, Cat. No. ABN1672, is a highly specific rabbit polyclonal antibody that targets HD-polyAla Ct and has been tested in Immunocytochemistry, Immunohistochemistry, and Western Blotting.

Immunohistochemistry Analysis: A representative lot detected HD-polyAla Ct in Immunohistochemistry applications (Banez-Coronel, M., et. al. (2015). Neuron. 88(4):667-77).

Western Blotting Analysis: A representative lot detected HD-polyAla Ct in Western Blotting applications (Banez-Coronel, M., et. al. (2015). Neuron. 88(4):667-77).

Immunocytochemistry Analysis: A representative lot detected HD-polyAla Ct in Immunocytochemistry applications (Banez-Coronel, M., et. al. (2015). Neuron. 88(4):667-77).

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected HD-polyAla Ct in pcDNA3.1 (Ctrl) & HEK293T-HD-polyAla-Ct.

Research Category
Neuroscience

品質

Evaluated by Western Blotting in HEK293T cells transfected with HD-polyAla-Ct.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected PolyAla in HEK293T cells transfected with HD-polyAla-Ct.

標靶描述

~15 kDa observed. Uncharacterized bands may be observed in some lysate(s).

外觀

Unpurified

Rabbit polyclonal antiserum with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable