跳转至内容
Merck
所有图片(1)

Key Documents

AB1784P

Sigma-Aldrich

Anti-Dopamine D1A Receptor Antibody, 4th Extracellular Domain

Chemicon®, from rabbit

登录查看公司和协议定价


About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

rabbit

品質等級

抗體表格

affinity purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

polyclonal

純化經由

affinity chromatography

物種活性

rat

製造商/商標名

Chemicon®

技術

ELISA: suitable
immunohistochemistry: suitable
western blot: suitable

NCBI登錄號

UniProt登錄號

運輸包裝

dry ice

目標翻譯後修改

unmodified

基因資訊

human ... DRD1(1812)

特異性

Dopamine D1A Receptor (D1R). The rat D1A receptor is a 446 amino acid, G-protein coupled transmembrane receptor protein (Monsma, 1990; Zhou, 1990; Zhou, 1992; O′Dowd, 1990). The immunogen sequence has 88% sequence similarity with human and 77% with porcine.

免疫原

A nine amino acid peptide corresponding to the 4th extracellular domain of the rat D1A receptor. The immunogen is absolutely conserved in the mouse D1A receptor and shows 88% homology with human and 77% with porcine and bovine D1A receptors. The peptide shows no significant sequence similarity with other dopamine receptors (D1B, D2-D5).
Epitope: 4th Extracellular Domain

應用

Detect Dopamine D1A Receptor using this Anti-Dopamine D1A Receptor Antibody, 4th Extracellular Domain validated for use in ELISA, IH & WB.
Immunohistochemistry: 2-10 μg/mL on paraformaldehyde fixed tissue sections. D1R has been localized in various regions of the rat brain (Huang, 1992; O′Connel, 1995)

Western blot: 1-10 μg/mL (Chemiluminescence technique). An antibody made to this peptide has been shown to detect 2 proteins of 50 and 75 kDa in solubilized hippocampal membranes. The 75 kDa band was not visible in striatum (Huang, 1992; O′Connel, 1995). Approximately 100-400 μg of protein/lane should be loaded to visualize the 75 kDa band (Huang, 1992; O′Connel, 1995) that is present in relatively small amounts.

ELISA: 0.5-1.0 μg/mL using 1 μg/mL of control peptide per well.

The immunogen peptide is available (Cat. No. AG266) for use in ELISA or antibody blocking studies. AG266 is not recommended for use as a western blot positive control.

Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors

外觀

Affinity purified immunoglobulin. Liquid in PBS with 0.2% BSA and 0.05% sodium azide.

儲存和穩定性

Maintain at -20°C in undiluted aliquots for up to 6 months from date of receipt. Avoid repeated freeze/thaw cycles.

分析報告

Control
POSITIVE CONTROL: rat striatal membranes (western blot)

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

未找到合适的产品?  

试试我们的产品选型工具.

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库

Modification of dopaminergic markers expression in the striatum by neonatal exposure to glutamate during development.
S J Lopez-Perez, P Vergara, J P Ventura-Valenzuela, M E Ure?a-Guerrero, J Segovia, C Beas-Zarate
International Journal of Developmental Neuroscience null
Schizophrenia: dopamine D1 receptor sequence is normal, but has DNA polymorphisms.
Ohara, K, et al.
Neuropsychopharmacology, 8, 131-135 (1993)
Glenn S Belinsky et al.
Stem cells and development, 22(10), 1522-1540 (2013-01-05)
We tested whether dopaminergic drugs can improve the protocol for in vitro differentiation of H9 human embryonic stem cells (hESCs) into dopaminergic neurons. The expression of 5 dopamine (DA) receptor subtypes (mRNA and protein) was analyzed at each protocol stage
Estrogen protects against the synergistic toxicity by HIV proteins, methamphetamine and cocaine.
Turchan, J; Anderson, C; Hauser, KF; Sun, Q; Zhang, J; Liu, Y; Wise, PM; Kruman et al.
BMC Neuroscience null
Tyler W Stradleigh et al.
Progress in retinal and eye research, 48, 181-202 (2015-04-22)
Immunohistochemical and ex vivo anatomical studies have provided many glimpses of the variety, distribution, and signaling components of vertebrate retinal neurons. The beauty of numerous images published to date, and the qualitative and quantitative information they provide, indicate that these

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门