推荐产品
生物源
sheep
品質等級
抗體表格
serum
抗體產品種類
primary antibodies
無性繁殖
polyclonal
物種活性
rat, human, chicken, mouse
應無反應活性
bovine
製造商／商標名
Chemicon®
技術
ELISA: suitable
immunohistochemistry: suitable
western blot: suitable
NCBI登錄號
UniProt登錄號
運輸包裝
dry ice
目標翻譯後修改
unmodified
基因資訊
human ... NGF(4803)
特異性
Nerve growth factor-beta (NGF). Less than 1% cross-reactivity against recombinant human NT4 and BDNF and approximately 2% for NT3 by ELISA.
免疫原
Mouse nerve growth factor-beta purified from salivary glands.
應用
Detect Nerve Growth Factor-β using this Anti-Nerve Growth Factor-β Antibody validated for use in ELISA, WB, IH.
Immunohistochemistry at 1:500-1:5,000 (see suggested protocol).
Immunoblotting at 1:500-1:5,000.
ELISA at 1:500-1:5,000.
Inhibition of biological activity in vitro at 1:10-1:50.
BIOLOGICAL ACTIVITY: Neutralizes NGF, but not other neurotrophins.
Use neat for in vivo animal studies (5-10 μL/g body weight).
Optimal working dilutions must be determined by end user.
Immunoblotting at 1:500-1:5,000.
ELISA at 1:500-1:5,000.
Inhibition of biological activity in vitro at 1:10-1:50.
BIOLOGICAL ACTIVITY: Neutralizes NGF, but not other neurotrophins.
Use neat for in vivo animal studies (5-10 μL/g body weight).
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurochemistry & Neurotrophins
Neurochemistry & Neurotrophins
外觀
Whole sheep antisera. Lyophilized. Reconstitute with 250 μL of sterile distilled water.
儲存和穩定性
Maintain at -20°C to -70°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.
法律資訊
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Hiroyuki Murota et al.
The Journal of allergy and clinical immunology, 130(3), 671-682 (2012-07-10)
Itch impairs the quality of life for many patients with dermatoses, especially atopic dermatitis (AD), and is frequently induced by a warm environment. To determine the mechanism underlying itch induction by warmth, we focused on artemin, a member of glial
D Joelson et al.
Microscopy research and technique, 41(3), 246-262 (1998-05-30)
The distribution of immunoreactivity for the ionotropic N-methyl-D-aspartate (NMDA) receptor subunits was mapped in the cochlear nucleus of postnatal day (P) 7, P14, P21, and P28 gerbils. Frozen sections and serial plastic sections of tissue were incubated with antibodies to
Daniel Aridgides et al.
PloS one, 8(2), e57450-e57450 (2013-02-26)
Cardiomyocytes express neurotrophin receptor TrkA that promotes survival following nerve growth factor (NGF) ligation. Whether TrkA also resides in cardiac fibroblasts (CFs) and underlies cardioprotection is unknown. To test whether CFs express TrkA that conveys paracrine signals to neighbor cardiomyocytes
X F Zhou et al.
Journal of neuroscience methods, 54(1), 95-102 (1994-09-01)
Nerve growth factor (NGF) is a survival factor required by a number of neuronal populations including most post-ganglionic sympathetic neurones. NGF has been detected and quantified in many tissues but there is little information regarding its cellular localization. Although it
I K Abrahamson et al.
Journal of neurocytology, 16(3), 417-422 (1987-06-01)
Immunohistochemistry has been used to demonstrate the presence of nerve growth factor (NGF)-like immunoreactivity in normal and sectioned mouse sciatic nerves. In normal nerves, immunoreactive material was not visible unless a silk ligature had previously been applied to constrict the
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