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Origami B(DE3) 感受态细胞 - Novagen

Escherichia coli, rod shaped

别名:

Competent cells, Novagen competent cells

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About This Item

分類程式碼代碼:
41106202
NACRES:
NA.81

product name

Origami B(DE3) 感受态细胞 - Novagen, Origami B host strains carry the same mutations as the original Origami strain, except that they are derived from a lacZY mutant of BL21 to enable precise control of expression levels using IPTG.

生物源

Escherichia coli

品質等級

100

製造商／商標名

Novagen®

儲存條件

OK to freeze

增長模式

adherent or suspension

形態學

rod shaped

技術

microbiological culture: suitable

細胞轉化

transformation efficiency: >2×106 cfu/μg

運輸包裝

dry ice

儲存溫度

−70°C

相关类别

一般說明

基因型：F-ompT hsdSB(rB- mB-) gal dcm lacY1 ahpC（DE3) gor522::Tn10 trxB（KanR，TetR)





该产品包含转基因生物(GMO)。在欧盟范围内，转基因物种分别受欧洲议会和理事会的2001/18 /EC和2009/41/EC指令及其成员国中国家所实施规范的约束。该法规规定我们有义务索取您以及处理转基因生物的机构的相关信息。请点击here获得最终用户声明（EUD）表格。
Origami B宿主菌株在trxB和gor中携带与原始Origami菌株相同的突变，只是它们来自BL21的lacZY突变体，可以通过调节IPTG的浓度来精确控制表达水平。因此Origami B菌株在一个菌株背景中结合了BL21、Tuner和Origami菌株的理想特征。trxB和gor的突变分别在卡那霉素和四环素上是可选择的；因此，这些菌株不能与只能用卡那霉素或四环素选择的质粒一起使用。这些菌株还包括BL21的lon和ompT缺陷，可增加蛋白质的稳定性。

DE3表明宿主是λDE3的溶原菌，因此在lacUV5启动子的控制下携带T7 RNA聚合酶基因的染色体拷贝。这样的菌株适用于通过用IPTG诱导从克隆在pET载体中的靶基因生产蛋白质。
Origami B宿主菌株携带与原始Origami菌株相同的突变，不同之处在于它们源自BL21的lacZY突变体，可以使用IPTG精确控制表达水平。

成分

0.4 ml1 ml组分

•2 × 0.2 ml5 × 0.2 mlOrigami B(DE3)感受态细胞

•2 × 2 ml4 × 2 mlSOC培养基

•2 ng2 ng供试质粒

警告

毒性：多个毒性值，请参阅MSDS（O）

法律資訊

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 2

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