生物源
mouse
品質等級
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
5C6, monoclonal
形狀
lyophilized
包含
≤0.1% sodium azide as preservative
物種活性
human, mouse
製造商／商標名
Calbiochem®
儲存條件
OK to freeze
同型
IgG1
運輸包裝
ambient
儲存溫度
−20°C
目標翻譯後修改
phosphorylation (pSer157)
基因資訊
human ... VASP(7408)
一般說明
Mouse monoclonal antibody purified from serum-free cell culture supernatant by thiophilic adsorption and size exclusion chromatography. Recognizes the the ~50 kDa VASP protein phosphorylated at Ser157.
Recognizes the the ~50 kDa VASP protein phosphorylated at Ser157.
This PhosphoDetect Anti-VASP (pSer¹⁵⁷) Mouse mAb (5C6) is validated for use in ELISA, Immunoblotting, ICC, IP for the detection of VASP (pSer¹⁵⁷).
免疫原
Human
a synthetic phosphopeptide corresponding to amino acids surrounding the Ser¹⁵⁷ phosphorylation site of human VASP
應用
ELISA (0.05 µg/ml)
Immunoblotting (1 µg/ml, colorimetric; 0.1 µg/ml, chemiluminescence)
Immunocytochemistry (1-10 µg/ml)
Immunoprecipitation (1-10 µg/10⁶ vanadate-treated A431 cells)
Immunoblotting (1 µg/ml, colorimetric; 0.1 µg/ml, chemiluminescence)
Immunocytochemistry (1-10 µg/ml)
Immunoprecipitation (1-10 µg/10⁶ vanadate-treated A431 cells)
警告
Toxicity: Standard Handling (A)
外觀
Lyophilized from PBS, PEG, sucrose.
重構
Reconstitute in 1 ml sterile, dH₂O, 15 min, room temperature. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot, flash-freeze in liquid nitrogen, and freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Avoid freeze/thaw cycles of solutions.
分析報告
Positive Control
Vanadate-treated A431 cells
Vanadate-treated A431 cells
其他說明
Bear, J.E., et al. 2001. Curr. Opin. Cell. Biol.13, 158.
Lawrence, D.W., and Pryzwansky, K.B. 2001. J. Immunol.166, 5550.
Burkhardt, M., et al. 2000. J. Biol. Chem.275, 33536.
Harbeck, B., et al. 2000. J. Biol. Chem.275, 40.
Smolenski, A., et al. 2000. J. Biol. Chem.275, 25723.
Lawrence, D.W., and Pryzwansky, K.B. 2001. J. Immunol.166, 5550.
Burkhardt, M., et al. 2000. J. Biol. Chem.275, 33536.
Harbeck, B., et al. 2000. J. Biol. Chem.275, 40.
Smolenski, A., et al. 2000. J. Biol. Chem.275, 25723.
Does not cross-react with non-phosphorylated VASP or with unrelated phosphorylation sites. For immunocytochemistry, fixation protocol may include up to 0.5% formaldehyde. Variables associated with assay conditions will dictate the proper working dilution.
法律資訊
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Min Li et al.
The Journal of biological chemistry, 290(50), 30030-30041 (2015-10-27)
Diabetes is strongly associated with cognitive decline, but the molecular reasons are unknown. We found that fasting and peripheral insulin promote phosphorylation and dephosphorylation, respectively, of specific residues on brain proteins including cytoskeletal regulators such as slit-robo GTPase-activating protein 3
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