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Merck
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主要文件

540221

Sigma-Aldrich

Purine Nucleoside Phosphorylase, Human, Recombinant, E. coli

别名:

PNP

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About This Item

分類程式碼代碼:
12352202
NACRES:
NA.42

重組細胞

expressed in E. coli

品質等級

形狀

liquid

比活性

≥25 units/mg protein

製造商/商標名

Calbiochem®

運輸包裝

dry ice

儲存溫度

−20°C

一般說明

Recombinant, human purine nucleotide phosphorylase expressed in E. coli without tags. Purine nucleoside phosphorylase catalyzes the phosphorlysis of N-ribosidic bonds of purine nucleosides and deoxynucleosides in a reversible reaction. Human PNP is a target for therapeutic T-cell immune response intervention. Useful in the detection of inorganic phosphate from biochemical reactions.
Recombinant, human purine nucleotide phosphorylase expressed in E. coli without tags. Purine nucleoside phosphorylase catalyzes the phosphorolysis of N-ribosidic bonds of purine nucleosides and deoxynucleosides. This reaction is reversible. The human enzyme is a target for therapeutic immune response intervention.

警告

Toxicity: Standard Handling (A)

單位定義

One unit is defined as the amount of enzyme that will convert 1 µmol of MESG and 1 µmol of Pi into 1 µmol of 2-amino-6-mercapto-7-methylpurine and 1 µmol of ribose phosphate in 1 min at 25°C, pH 7.6.

外觀

In 50 mM Tris-HCl, pH 7.6.

分析報告

Single band by SDS-PAGE

其他說明

Filgueira de Azevedo, W.Jr., et al. 2003. Biochem. Biophys. Res. Commun.309, 917.
Silva, R.G., et al. 2003. Protein Expr. Purif.27, 158.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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