539578

PKG, Iα, Bovine Lung

• 别名: cGMP-Dependent Protein Kinase Catalytic Subunit, PKG _Iα Catalytic Subunit, Protein Kinase G Iα, cGMP-Dependent Protein Kinase Catalytic Subunit, PKG_Iα Catalytic Subunit, Protein Kinase G Iα
• 分類程式碼代碼: 12352202
• NACRES: NA.42

属性

• 形狀: liquid
• 品質等級: 100
• 比活性: ≥1300 units/μg protein
• 製造商／商標名: Calbiochem^®
• 儲存條件: OK to freeze; avoid repeated freeze/thaw cycles
• 運輸包裝: wet ice
• 儲存溫度: −70°C

说明

一般說明

Note: 4µg = 6000 units.

Native PKGIα purified from bovine lung. PKG is involved in regulation of smooth muscle relaxation, platelet function, and cell division. PKG is activated by binding of cGMP to regulatory subunits within the holoenzyme, but in contrast to PKA, the activation of PKG does not invlove physical dissociation of catalytic from the regulatory domains. The activated enzyme phosphorylates target proteins at serine and/or threonine residues that are found within the recognition motif, Arg-Lys-Arg-Ser*-Arg-Ala-Glu.

包裝

Please refer to vial label for lot-specific concentration.

警告

Toxicity: Standard Handling (A)

單位定義

One unit is defined as the amount of enzyme required to incorporote 1 pmol phosphate into the synthetic heptapeptide substrate, RKRSRAE, in 1 min at 30°C.

外觀

In 150 mM NaCl, 25 mM β-mercaptoethanol, 10 mM K₂HPO₄, 1 mM EDTA, 12% sucrose, pH 6.8.

重構

Following initial thaw, aliquot and freeze (-70°C).

其他說明

Brophy, M.C., et al. 2006. J. Vasc. Res.39, 95.
Colbran J.L., et al. 1992. J. Biol. Chem.267, 9589.
Corbin, J.D. and Doskeland, S.O. 1983. J Biol Chem258, 11391.
Witt, J.J. and Roskoski, R., Jr, 1975. Anal Biochem66, 253.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全信息

• 象形圖: GHS07
• 訊號詞: Warning
• 危險聲明: H317
• 防範說明: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• 危險分類: Skin Sens. 1
• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable