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Merck

C8482

Sigma-Aldrich

Protein Kinase A catalytic subunit human

>90% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution

别名:

Cyclic AMP-dependent protein kinase catalytic subunit, histidine-tagged, recombinant human

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About This Item

MDL號碼:
分類程式碼代碼:
41106305
NACRES:
NA.32

重組細胞

expressed in E. coli

品質等級

化驗

>90% (SDS-PAGE)

形狀

buffered aqueous glycerol solution

比活性

>1000 units/mg protein

分子量

43.5 kDa

UniProt登錄號

運輸包裝

dry ice

儲存溫度

−70°C

基因資訊

human ... PRKACA(5566)

生化/生理作用

Ubiquitous serine-threonine kinase that phosphorylates a broad spectrum of substrates, and regulates many cellular processes. The catalytic subunit is released following binding of cyclic AMP to the regulatory subunits of the PKA holoenzyme. The free catalytic subunit has intrinsic activity and does not require added cyclic AMP.

外觀

Solution in 30 mM potassium phosphate buffer, pH 7.4, containing 50% glycerol, 150 mM KCl, 1 mM EDTA, and 1 mM DTT.

分析報告

One unit will transfer 1 nanomole of phosphate from 32P-ATP to kemptide substrate per minute at pH 7.4 at 30 °C (measured by a radioactive filter-binding assay).
Km (ATP) = 25 μM at 30 °C, pH 7.4. Km (kemptide) = 42 μM (33 μg/ml) at 30 °C, pH 7.4.

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Toshio Kitazawa et al.
The Journal of physiology, 587(Pt 14), 3587-3603 (2009-05-28)
Nitric oxide (NO) from endothelium is a major mediator of vasodilatation through cGMP/PKG signals that lead to a decrease in Ca(2+) concentration. In addition, NO-mediated signals trigger an increase in myosin light chain phosphatase (MLCP) activity. To evaluate the mechanism
Chung-Lin Chou et al.
American journal of physiology. Cell physiology, 314(1), C99-C117 (2017-10-20)
The urea channel UT-A1 and the water channel aquaporin-2 (AQP2) mediate vasopressin-regulated transport in the renal inner medullary collecting duct (IMCD). To identify the proteins that interact with UT-A1 and AQP2 in native rat IMCD cells, we carried out chemical
Tadahiko Matsumoto et al.
Scientific reports, 9(1), 8307-8307 (2019-06-06)
APOBEC3B cytidine deaminase (A3B) catalyzes cytosine into uracil in single-strand DNA and induces C-to-T mutations in genomic DNA of various types of tumors. Accumulation of APOBEC signature mutations is correlated with a worse prognosis for patients with breast cancer or

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