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524627

Millipore

Phosphatase Inhibitor Cocktail III

liquid, for the inhibition of serine/threonine and protein tyrosine phosphatases

别名:

磷酸酶抑制剂混合物(套装III)

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About This Item

分類程式碼代碼:
12352200
NACRES:
NA.77

product name

磷酸酶抑制剂混合物套装III, Phosphatase Inhibitor Cocktail Set III is a ready to use cocktail of four phosphatase inhibitors for broad-spectrum inhibition of both serine/threonine and protein tyrosine phosphatases.

形狀

liquid

製造商/商標名

Calbiochem®

儲存條件

OK to freeze

運輸包裝

wet ice

儲存溫度

−20°C

一般說明

磷酸化和去磷酸化可调节细胞分裂、增殖和凋亡等细胞活动的信号转导过程。蛋白激酶在目标蛋白上的特定氨基酸残基(丝氨酸、苏氨酸或酪氨酸)上添加磷酸基团,改变它们的构象和与配体的相互作用。蛋白质磷酸酶去除磷酸基团,使蛋白质恢复到去磷酸化状态。当从细胞和组织裂解物中提取磷酸化蛋白时,必须保持其磷酸化状态。磷酸酶抑制剂混合物组III是四种磷酸酶抑制剂的混合物,用于广谱抑制丝氨酸/苏氨酸和蛋白质酪氨酸磷酸酶。可以1 ml小瓶或一组5个1 ml小瓶的形式提供。

應用

磷酸酶抑制剂混合物套装III已用于:
  • 作为裂解缓冲液的补充,制备全睾丸蛋白裂解液,进行蛋白质印迹分析
  • 强化冷藏裂解放射免疫沉淀(RIPA)缓冲液,均质化幼虫后肠,以进行蛋白质印迹分析
  • 作为细胞裂解缓冲液的成分,提取总蛋白用于蛋白质印迹分析

生化/生理作用

主要靶标
丝氨酸/苏氨酸和蛋白质酪氨酸磷酸酶
产物不与ATP竞争。
可逆:否
细胞渗透性:否

特點和優勢

  • 方便的即用型配方
  • 高品质的产品,具有一致和可重复的性能
  • 灵活选择一系列针对不同类别磷酸酶的混合物

成分

每瓶含有1 ml水溶液,含有以下成分:
  • 50 mM氟化钠
  • 10 mMß-甘油三酯(目录号35675)
  • 10 mM十水焦磷酸钠
  • 1 mM正钒酸钠

警告

毒性:有毒(F)

外觀

在H₂O中。

重構

为了长期储存,分装冻存(-20°C)。临用前按1:5稀释。

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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