524627
磷酸酶抑制剂混合物套装III
Phosphatase Inhibitor Cocktail Set III is a ready to use cocktail of four phosphatase inhibitors for broad-spectrum inhibition of both serine/threonine and protein tyrosine phosphatases.
别名:
Phosphatase Inhibitor Mix (Set III), Phosphatase inhibitor
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所有图片(1)
About This Item
分類程式碼代碼:
12352200
NACRES:
NA.77
推荐产品
形狀
liquid
製造商／商標名
Calbiochem®
儲存條件
OK to freeze
運輸包裝
wet ice
儲存溫度
−20°C
相关类别
一般說明
Phosphorylation and dephosphorylation regulate signal transduction processes involved in cellular activities such as cell division, proliferation, and apoptosis. Protein kinases add a phosphate group to specific amino acid residues (serine, threonine, or tyrosine) on target proteins, changing their conformation and interaction with ligands. Protein phosphatases remove the phosphate group, restoring the protein to its dephosphorylated state. When extracting phosphorylated proteins from cell and tissue lysates, it is crucial to maintain their phosphorylation status intact. Phosphatase Inhibitor Cocktail Set III is a cocktail of four phosphatase inhibitors for broad-spectrum inhibition of both serine/threonine and protein tyrosine phosphatases. Available as a 1 ml vial or as a set of five 1 ml vials.
應用
Phosphatase Inhibitor Cocktail Set III has been used:
- as a supplement in lysis buffer to prepare protein lysates of whole testes for western blotting
- to fortify ice-cold lysis radio-immunoprecipitation assay (RIPA) buffer to homogenize larval hindguts for western blot analysis
- as a component in cell lysis buffer to extract total proteins for western blotting
生化／生理作用
主要靶标
丝氨酸/苏氨酸和蛋白质酪氨酸磷酸酶
丝氨酸/苏氨酸和蛋白质酪氨酸磷酸酶
产物不与ATP竞争。
可逆：否
细胞渗透性：否
特點和優勢
- Ready-to-use formulations for convenient use
- High-quality products for consistent and reproducible performance,
- Flexible options with a range of cocktails targeting different categories of phosphatases
成分
Each vial contains 1 ml of aqueous solution with the following components:
- 50 mM Sodium Fluoride
- 10 mM ß-Glycerophosphate (Cat. No. 35675)
- 10 mM Sodium Pyrophosphate Decahydrate
- 1 mM Sodium Orthovanadate
警告
毒性：有毒（F）
外觀
在H₂O中。
重構
为了长期储存，分装冻存（-20°C）。临用前按1：5稀释。
法律資訊
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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