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产品名称
磷酸酶抑制剂混合物组合II, A cocktail of five phosphatase inhibitors for the inhibition of acid and alkaline phosphatases as well as protein tyrosine phosphatases (PTPs). Suitable for use with cell lysates and tissue extracts.
品質等級
形狀
liquid
製造商/商標名
Calbiochem®
儲存條件
OK to freeze
運輸包裝
wet ice
儲存溫度
−20°C
一般說明
五种磷酸酶抑制剂的混合物,可抑制酸和碱性磷酸酶以及蛋白质酪氨酸磷酸酶(PTP)。适用于细胞裂解液和组织提取物,包括含有去污剂的样品。
應用
磷酸酶抑制剂混合物套装III已用于:
- 在三倍洗涤剂缓冲液中裂解培养细胞,提取总蛋白用于蛋白质印迹分析
- 作为裂解缓冲液的组成部分、裂解组织样品或培养的大鼠心室心肌细胞(VCM),用于心肌线粒体蛋白的蛋白质印迹分析
- 作为裂解缓冲液的组成部分,裂解感染Adv-COX7RP和Adv-shRNA COX7RP的培养大鼠VCM,用于免疫沉淀莱诺二氏受体2型(RyR2)
- 作为冰冻RIPA缓冲液中的一种成分,匀质化/裂解视网膜组织,用于蛋白提取和蛋白质印迹分析
生化/生理作用
蛋白磷酸化和去磷酸化在调节细胞分裂、增殖和凋亡等多种细胞功能的信号转导过程中起重要作用。蛋白激酶将磷酸基团转移到特定的丝氨酸、苏氨酸或酪氨酸残基上,导致蛋白质的构象改变并影响其与配体的相互作用。蛋白质磷酸酶通过去除磷酸基团并将蛋白质恢复到其原始的去磷酸化状态来逆转这一过程。当从细胞和组织裂解物中提取磷酸化蛋白时,必须保持蛋白质的磷酸化状态。
特點和優勢
- 灵活:与细胞裂解液和组织提取物兼容,包括含有洗涤剂的样品。
- 经济:即用格式最大限度地减少浪费。
- 有效:1 ml足够约100 ml提取缓冲液。
- 完整:包含五种磷酸酶抑制剂,靶向酸性和碱性磷酸酶和蛋白酪氨酸磷酸酶(PTP)。
成分
提供一组5个1 ml小瓶,含有以下成分
- 咪唑(200 mM)
- 氟化钠(100 M)
- 钼酸钠(115mM)
- 正钒酸钠(100mM)
- 酒石酸钠二水合物(400mM)
警告
毒性:标准处理(A)
其他說明
由于该运输中有害物质的性质,您的订单可能需要支付额外的运输费用。某些尺寸的产品可免除其他有害材料的运输费用。请与您当地的销售办事处联系,以获取有关这些费用的更多信息。
法律資訊
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
訊號詞
Danger
危險聲明
危險分類
Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
儲存類別代碼
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
水污染物質分類(WGK)
WGK 2
閃點(°F)
Not applicable
閃點(°C)
Not applicable
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