推荐产品
品質等級
化驗
≥98% (HPLC)
形狀
solution
製造商/商標名
Calbiochem®
儲存條件
OK to freeze
protect from light
顏色
pale yellow
螢光
λex ~500 nm
λem ~515 nm
儲存溫度
−20°C
InChI
1S/C25H18F2N2O7/c1-10(30)33-20-8-18-13(6-15(20)26)25(12-4-5-17(29-3)23(28)22(12)24(32)36-25)14-7-16(27)21(34-11(2)31)9-19(14)35-18/h4-9,29H,28H2,1-3H3
InChI 密鑰
BEVHTVRRVVEMEF-UHFFFAOYSA-N
一般說明
A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM. Intracellularly releases DAF-FM (Cat. No. 251515) by the action of esterases. Reacts with NO, in the presence of O2, resulting in a triazolo-fluorescein analog (DAF-FM T) that exhibits about 160-fold greater fluorescence quantum efficiency. The fluorescence is reported to be stable above pH 5.8.
A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM. Releases DAF-FM (Cat. No. 251515) by the action of intracellular esterases. Reacts with NO, in the presence of oxygen, resulting in the formation of a triazolo-fluorescein analog (DAF-FM T) that exhibits about 160-fold greater fluorescence quantum efficiency. The fluorescence intensity is reported to be stable above pH 5.8. Excitation max.: ~500 nm; emission max.: ~515 nm.
生化/生理作用
Primary Target
Nitric oxide (NO) fluorescent indicator
Nitric oxide (NO) fluorescent indicator
警告
Toxicity: Irritant (B)
外觀
A 5 mM solution in DMSO.
重構
Following initial thaw, aliquot and freeze at (-20°C). Use immediately after dilution; discard any unused portion.
其他說明
Kojima, H., et al. 2001. J. Neurochem.76, 1404.
Park. J.M., et al. 2001. Br. J. Pharmacol.132, 1876.
Itoh, Y., et al. 2000. Anal. Biochem.287, 203.
Kojima, H., et al. 1999. Angew Chem. Int. Ed.38, 3209.
Park. J.M., et al. 2001. Br. J. Pharmacol.132, 1876.
Itoh, Y., et al. 2000. Anal. Biochem.287, 203.
Kojima, H., et al. 1999. Angew Chem. Int. Ed.38, 3209.
法律資訊
Manufactured by Daiichi Pure Chemicals Co., Ltd
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
儲存類別代碼
10 - Combustible liquids
水污染物質分類(WGK)
WGK 2
閃點(°F)
188.6 °F - closed cup - (Dimethylsulfoxide)
閃點(°C)
87 °C - closed cup - (Dimethylsulfoxide)
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