推荐产品
品質等級
物種活性
human, rat, mouse
製造商／商標名
Upstate®
技術
activity assay: suitable
affinity binding assay: suitable
UniProt登錄號
運輸包裝
dry ice
基因資訊
human ... RAC1(5879)
一般說明
非放射性Rac1活化测定试剂盒可用于沉淀细胞裂解物中的Ras相关C3肉毒杆菌毒素底物1 (Rac1)-GTP，并通过Rac1特异性单克隆抗体检测。Rac1属于小鸟苷三磷酸酶（GTPases）的Ras超级家族。它以两种主要形式表达，即非活性GDP结合形式和活性GTP结合形式。Rac1通过与激活多种信号级联的效应物相互作用，参与影响多种生理条件。RAC1基因位于人染色体7p22.1。
應用
Rac1激活测定试剂盒已用于通过免疫印迹法检测小鼠和人脑匀浆中的活性Ras相关C3肉毒杆菌毒素底物1 (Rac1)。
成分
抗Rac1，克隆23A8（目录号05-389）
Mg2+裂解/洗涤缓冲液，5X（目录号20-168）
100X GTPγS，10mM（目录号20-176）
100X GDP，100mM（目录号20-177）
Rac/Cdc42测定试剂（PAK1 PBD，琼脂糖）（目录号14-325）
Mg2+裂解/洗涤缓冲液，5X（目录号20-168）
100X GTPγS，10mM（目录号20-176）
100X GDP，100mM（目录号20-177）
Rac/Cdc42测定试剂（PAK1 PBD，琼脂糖）（目录号14-325）
品質
通过从已加载GTPγS的3T3细胞裂解物中沉淀Rac-GTP进行常规评估。 使用抗Rac通过免疫印迹分析检测沉淀的Rac-GTP。
聯結
替代：SGT425
法律資訊
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
訊號詞
Danger
危險聲明
危險分類
Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1
儲存類別代碼
10 - Combustible liquids
Julie A Cook et al.
The Journal of biological chemistry, 278(37), 35812-35818 (2003-07-05)
Activation of HIV-1-infected T cells through the T cell receptor and costimulatory molecule CD28 induces proviral transcription; however, the mechanism behind this enhanced virus expression is unknown. Jurkat T cells and primary CD4+ T cells expressing a CD8 alpha/CD28 chimeric
Elizabeth A Pickett et al.
Development (Cambridge, England), 135(3), 589-598 (2008-01-15)
Spina bifida, or failure of the vertebrae to close at the midline, is a common congenital malformation in humans that is often synonymous with neural tube defects (NTDs). However, it is likely that other etiologies exist. Genetic disruption of platelet-derived
Anti-arrhythmic surgery for atrioventricular junctional and atrioventricular re-entrant tachycardia--a report of six cases.
Koo, C C, et al.
Annals of the Academy of Medicine, Singapore, 19, 67-72 (1990)
Michelle E Marchese et al.
Biochemistry, 51(41), 8235-8246 (2012-09-14)
Vascular adhesion molecules regulate the migration of leukocytes from the blood into tissue during inflammation. Binding of leukocytes to vascular cell adhesion molecule-1 (VCAM-1) activates signals in endothelial cells, including Rac1 and calcium fluxes. These VCAM-1 signals are required for
Incorporation of CXCR4 into membrane lipid rafts primes homing-related responses of hematopoietic stem/progenitor cells to an SDF-1 gradient.
Marcin Wysoczynski, Ryan Reca, Janina Ratajczak, Magda Kucia, Neeta Shirvaikar et al.
Blood null
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门