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Key Documents

1.50617

Supelco

Purospher®STAR RP-18 endcapped (5 µm) HPLC Columns

L × I.D. 150 mm x 3 mm Hibar® RT HPLC Column

别名:

Purospher® STAR RP-18 endcapped 5µm Hibar® RT 150-3 HPLC column

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About This Item

分類程式碼代碼:
41115709
NACRES:
SB.52

product name

Purospher®STAR RP-18 封端(5 µm), L × I.D. 150 mm x 3 mm Hibar® RT HPLC column

材料

stainless steel column

品質等級

agency

suitable for USP L1

產品線

Purospher®

特點

endcapped

包裝

1 ea of

標籤範圍

17% Carbon loading

參數

400 bar pressure
65 °C max. temp.

技術

HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (High)

柱長度 × 內徑

15 cm × 3 mm

表面積

330 m2/g

基質

fully porous particle

基質活性組

C18 bonding phase

粒徑

5 μm

孔徑

120 Å pore size

工作pH值範圍

1.5-10.5

分離技術

reversed phase
supercritical fluid (SFC)

儲存溫度

no temp limit

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一般說明

Purospher® STAR RP-18 endcapped, 5 µm HPLC columns are designed for universal use and allow the separation of basic, neutral, and metal chelating compounds with simple mobile phases and excellent peak symmetry. They are based on ultra-pure fully porous silica particles (Type B). These columns offer an outstanding stability from pH 1.5 to 10.5 over a wide temperature range, and suitability for up to 100% aqueous mobile phases. Plus, they demonstrate best all-round retention characteristics, as proven by the Tanaka test. Thanks to these features, Purospher® STAR RP-18 endcapped, 5 µm HPLC columns allow maximum flexibility to use the best chromatographic conditions for any separation including reversed phase.Purospher® STAR RP-18 endcapped, 5 µm Hibar® RT 150-3 is a ready to use stainless steel column suitable for connection to any HPLC system.The column dimension of 150 mm length x 3 mm I.D. is the optimal choice for the high resolution HPLC separation of complex samples , allowing significant solvent savings and improved sensitivity. A 3 mm I.D. column enables a more sustainable separations in comparison to 4 or 4.6 mm I.D. column due to the use of less solvent for the chromatographic separation.The recommended guard column is Purospher® STAR RP-18 endcapped (5 µm) LiChroCART 4-4 part number 1.50250.0001. For direct connection to a Hibar® RT columns the Precolumn holder for 4-4 LiChroCART cartridges, part number 1.51487.0001 can be used.Item 1.50617.1003 is a Validation kit containing 3 Purospher® STAR RP-18 endcapped, 3 µm Hibar® RT 150-3 HPLC columns from 3 different base material lots.Learn more about Purospher® STAR HPLC and UHPLC ColumnsLearn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection GuideBrochure: A Practical Guide to HPLC - Method Development Guide

應用

Intended to be used in the separation of aflatoxins, aldehydes, alkaloids, antibiotics, catecholamines, explosives, oligonucleotides, esters, fat soluble vitamins, lipids, fatty acids, flavonoids, ketones, nitrosamines, nucleosides, PAHs, PCBs, peptides, pesticides, phenols, phthalates, preservatives, steroids, metabolized steroids, sulfonamides, sweeteners, and water soluble vitamins.

特點和優勢

  • Ultra-pure silica (99.999%) for excellent peak symmetry
  • High separation efficiency
  • Reproducible results from run-to-run and batch-to-batch
  • Best all-round performance according to Tanaka
  • Outstanding pH stability from pH 1.5 to 10.5
  • No phase collapse when using highly aqueous mobile phases
  • Good suitability for LC-MS applications

分析報告

Pressure: ≤ 80 bar
Theoretical Plates (N/m) (Toluene): ≥ 80000
Symmetry (Toluene): 0.8 - 1.4
Capacity factor (Toluene): 1.0 - 3.0

法律資訊

Hibar is a registered trademark of Merck KGaA, Darmstadt, Germany
LICHROCART is a trademark of Merck KGaA, Darmstadt, Germany
Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany

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