1.50448

SeQuant^™ ZIC^®-HILIC 200Å (3.5 µm) HPLC Columns

L × I.D. 150 mm x 2.1 mm HPLC Column, PEEK-lined stainless steel

• 别名: Zwitterionic Hydrophilic Interaction Liquid Chromatography HPLC Column
• 分類程式碼代碼: 41115709

属性

• product name: SeQuant^™ ZIC-HILIC^™ 3.5µm,200Å 150 x 2.1 mm, PEEK-lined stainless steel column, suitable for HPLC
• 材料: PEEK-lined stainless steel column
• 品質等級: 100
• agency: suitable for USP L114
• 產品線: SeQuant^™
• 特點: endcapped: no
• 參數: 550 bar pressure; 70 °C max. temp.
• 技術: HPLC: suitable; LC/MS: suitable; UHPLC-MS: suitable; UHPLC: suitable; mass spectrometry (MS): suitable (High)
• 柱長度 × 內徑: 15 cm × 2.1 mm
• 基質: fully porous particle
• 基質活性組: ZIC-HILIC bonding phase
• 粒徑: 3.5 μm
• 孔徑: 200 Å pore size
• 工作pH值: 2-7.5
• 分離技術: hydrophilic interaction (HILIC)
• 儲存溫度: no temp limit

说明

一般說明

SeQuant^™ ZIC^®-HILIC色谱柱旨在保留和分离所有类型的极性和亲水化合物，实现高选择性和可重复性的稳健色谱。 硅基ZIC^®-HILIC吸附剂具有由高极性永久两性离子组成的键合固定相。1:1的两性离子电荷平衡有利于分离选择性，使得ZIC^®-HILIC柱整体呈中性，但具有微弱而重要的离子相互作用。在方法开发过程中，ZIC^®-HILIC色谱柱的选择性调整是由与pH无关的永久两性离子促进的，从而确保在洗脱液优化过程中只有分析物(而不是色谱柱)受到影响。200 Å的孔径使该色谱柱非常适合在HILIC条件下分离小极性分子。色谱柱尺寸为150 mm长x 2.1 mm ID，非常适合使用UHPLC或优化的HPLC仪器分离极性化合物，从而提供低驻留体积。由于良好的保留和强大的性能，SeQuant^™ HILIC色谱柱在世界各地的许多实验室中广受欢迎。例如，美国食品和药物管理局(FDA)推荐SeQuant^™ ZIC^®-HILIC用于分析三聚氰胺及相关物质。SeQuant^™ HILIC色谱柱具有稳定性和低流失率。这些特点使它们特别适用于LC-MS应用，其中高底噪会导致信号抑制和定量测量干扰，同时也会增加仪器磨损。它们的高稳定性使得这些高性能的HILIC色谱柱对于传统的HPLC应用也很有吸引力。了解更多关于Supelco HPLC色谱柱组合和方法开发的信息：手册：HPLC和UHPLC色谱柱选择指南 手册：HPLC实践指南 - 方法开发指南

應用

• Click postsynthesis of microporous organic network@silica composites: Demonstrates the application of SeQuant ZIC-HILI columns in mixed-mode chromatography, particularly in the synthesis of composites that facilitate both reversed-phase and hydrophilic interaction chromatography. This approach is crucial for advanced pharmaceutical compound analysis and biomolecule separation technology (Sun HF et al., 2023).


• Evaluation of Hold-Up Volume Determination Methods and Markers in Hydrophilic Interaction Liquid Chromatography: Focuses on refining the precision in volume determinations using ZIC-HILIC columns, enhancing method development for pharmaceuticals and providing a basis for high-resolution mass spectrometry applications (Redon L et al., 2023).


• Determination of the polyphenolic content of berry juices: Utilizes SeQuant ZIC-HILIC technology for comprehensive two-dimensional liquid chromatography, essential for complex sample analysis in food safety and quality assessments (Arena K et al., 2023).


• Simple and sensitive determination of sulfites in Chinese herbal teas: Highlights the robustness of SeQuant ZIC-HILIC in ultra-high-performance liquid chromatography tandem mass spectrometry, ensuring the safety and compliance of herbal products with stringent health standards (Yang L et al., 2022).

分析報告

理论塔板数（N/m）（胞嘧啶）： ≥ 90000
对称性（胞嘧啶）：0.95 - 1.40

法律資訊

SeQuant is a trademark of Merck KGaA, Darmstadt, Germany

ZIC is a registered trademark of Merck KGaA, Darmstadt, Germany

ZIC-HILIC is a trademark of Merck KGaA, Darmstadt, Germany