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Merck
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Key Documents

1.09217

Sigma-Aldrich

Gram′s safranine solution

for the Gram staining method

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About This Item

分類程式碼代碼:
41116130
NACRES:
NA.74

品質等級

形狀

liquid

IVD

for in vitro diagnostic use

技術

microbe id | staining: suitable

轉變溫度

flash point 49 °C

密度

0.98 g/cm3 at 20 °C

應用

clinical testing
diagnostic assay manufacturing
hematology
histology

儲存溫度

15-25°C

一般說明

This ready-to-use Gram′s safranine solution-for the Gram staining method, is used in bacteriology. The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram′s crystal violet solution (Product number 1.09218) - an aniline dye - in the first step. After the treatment with iodinesolution (Lugol′s solution stabilized with PVP, Product number 1.00567), a dye-iodine complex will form. During the decolorizing step (Gram′s decolorizing solution, 1.10218), this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue-violet. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized . Gram-negative bacteria will be counterstained by Gram′s safranin solution and appear pink to red.
A bottle with 500 ml Gram′s safranin solution is sufficient for staining up to 250 slides, it is also available as part of the Gram-color stain set, (Product number 1.11885). It is an IVD product and CE registered. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

象形圖

Flame

訊號詞

Warning

危險聲明

危險分類

Flam. Liq. 3

儲存類別代碼

3 - Flammable liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

120.2 °F

閃點(°C)

49 °C


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