07-500

Anti-p47-phox (mouse) Antibody

Upstate^®, from rabbit

• 别名: Cyclin-dependent kinase inhibitor p27, cyclin-dependent kinase inhibitor 1B, cyclin-dependent kinase inhibitor 1B (p27, Kip1)
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

属性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 物種活性: mouse
• 製造商／商標名: Upstate^®
• 技術: western blot: suitable
• 同型: IgG
• NCBI登錄號: NP_000256
• UniProt登錄號: Q09014
• 運輸包裝: dry ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... CDKN1B(1027)

说明

一般說明

Activation of NADPH-oxidase dependent superoxide generation requires the participation of several cytosolic factors including p47phox, p67phox, p40phox and Rac (1 or 2). In the resting cell, p47phox, p67phox, and p40phox form a large molecular weight complex which can be recovered from the cytosolic fraction. Activation of the NADPH oxidase is initiated by the assembly of p47phox, p67phox, and Rac with cytochrome b558 in a 1:1:1:1 complex in the plasma membrane. p47phox, p67phox, and Rac exhibit cooperative binding, since increasing the concentration of any one component lowers the EC50 of the other components. A great deal of current research involves understanding the protein protein interactions, and how these change with the activation state.

特異性

Recognizes mouse p47-phox, MW ~47 kDa.

免疫原

KLH-conjugated, synthetic peptides containing the first 15 amino acids of the N-terminal and the last 15 amino acids of the C-terminus of mouse p47-phox.

應用

Research Category
Neuroscience

Research Sub Category
Oxidative Stress

This Anti-p47-phox (mouse) Antibody is validated for use in WB for the detection of p47-phox (mouse).

品質

Evaluated by western blot in RIPA lysates from RAW 264.7 cells.

Western Blot Analysis:
0.5-2.0 µg/mL of this antibody detected p47-phox in RIPA lysates from RAW 264.7 cells.

標靶描述

~47 kDa

聯結

Replaces: 04-240

外觀

Protein A purified

Format: Purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide before the addition of glycerol to 30%.

儲存和穩定性

Stable for 1 year at -20ºC from date of receipt.

分析報告

Control
RAW 264.7 cell lysate.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律資訊

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 1