07-024

Anti-gp91-phox Antibody

Upstate^®, from rabbit

• 别名: Cytochrome b(558) subunit beta, Cytochrome b558 subunit beta, Heme-binding membrane glycoprotein gp91phox, NADPH oxidase, Neutrophil cytochrome b 91 kDa polypeptide, Superoxide-generating NADPH oxidase heavy chain subunit, chronic granulomatous disease,
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

属性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 物種活性: human
• 製造商／商標名: Upstate^®
• 技術: western blot: suitable
• 同型: IgG
• NCBI登錄號: NM_000397
• UniProt登錄號: P04839
• 運輸包裝: dry ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... CYBB(1536)

说明

一般說明

Cytochrome b-245, beta polypeptide (chronic granulomatous disease), also known as CYBB and P91-PHOX, is a human gene encoding a glycoprotein.
Cytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles.

特異性

Detects gp91-phox, Mr 75 kDa. Depending on the type of lysate, the amount loaded, the gel and the blotting conditions, gp91-phox may run as a sharp band, Mr 75 kDa, or appear as a smear between 75-91 kDa.

Predicted to cross react with mouse.

免疫原

KLH-conjugated, synthetic peptide corresponding to amino acids 548-560 of human gp91-phox (KQSISNSESGPRG). The immunizing sequence is identical in mouse, and it shares 11/13 sequence homology in bovine and 10/13 in porcine.

應用

Anti-gp91-phox Antibody is an antibody against gp91-phox for use in WB.

Immunoprecipitation:
Not recommended.

Research Category
Neuroscience

Research Sub Category
Oxidative Stress

品質

Routinely evaluated by Western Blot on human neutrophil membranes.

Western Blot Analysis:
1-4 µg/mL of this lot detected gp91-phox in a HL60 membrane and RIPA cell lysate. A previous lot detected gp91-phox in human neutrophil membranes.

標靶描述

Varies. Depending on the type of lysate, the amount loaded, the gel and the blotting conditions, gp91-phox may run as a sharp band, Mr ~75 kDa, or appear as a smear between 75-91 kDa

外觀

Protein A purified

Format: Purified

Purified rabbit polyclonal IgG in buffer containing 0.07 M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide and 30% glycerol. Liquid at -20°C.

儲存和穩定性

Stable for 1 year at -20°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

分析報告

Control
Lung tissue, human neutrophil membrane, HL60 membrane and RIPA cell lysate.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律資訊

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 1