推荐产品
生物源
mouse
品質等級
抗體表格
ascites fluid
抗體產品種類
primary antibodies
無性繁殖
9E8.2, monoclonal
物種活性
human
製造商/商標名
Upstate®
技術
ChIP: suitable (ChIP-seq)
western blot: suitable
同型
IgG1κ
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... AGO2(27161)
一般說明
Ago2(argonaute-2),也称为真核翻译起始因子2C(EIF2C2),是siRNA定向RNA干扰(RNAi)反应的重要组成部分。 Ago2是解开siRNA双链体和将siRNA组装成RNA诱导的沉默复合物(RISC)所需的核酸内切酶。 Ago2通过其Piwi域与DICER1相互作用。 该Piwi结构域被认为通过类似于RNase H的机制提供RNA切割活性。Ago2活性对于胚胎发育以及RNA介导的基因沉默(RNAi)是必需的。
特異性
人Ago2
免疫原
KLH偶联的合成肽,包含序列YSGAGPALAPPAPPPPIQG
表位:在Ago2的N端附近
應用
ChIP和ChIP-seq:该抗体的代表性批次用于执行ChIP和ChIP-seq(Woolnough, JL, Atwood, BL, and Giles KE (2015), MCB Vol. 35 No. 13, p. 2278-2294)。
抗Ago2抗体,克隆9E8.2是一种小鼠单克隆抗体,用于检测Ago2(也称为Argonaute-2,真核翻译起始因子2C),&已在WB中验证,并已证明可在ChIP和ChIP-seq中执行。
研究子类别
RNA代谢&结合蛋白
染色质生物
RNA结合蛋白(RBP)
RNA代谢&结合蛋白
染色质生物
RNA结合蛋白(RBP)
研究类别
表观遗传学&核功能
表观遗传学&核功能
品質
已通过免疫印迹进行常规评估。
標靶描述
~100 kda
外觀
免疫亲和纯化
含0.05%叠氮化钠的腹水
儲存和穩定性
自收到之日起,在-20°C条件下可稳定保存1年。
处理建议:收到后,在取下瓶盖之前,将小瓶离心并轻轻混合溶液。分装到微量离心管中,并储存于 -20°C。避免反复冻融循环,以免损坏IgG和影响产品性能
处理建议:收到后,在取下瓶盖之前,将小瓶离心并轻轻混合溶液。分装到微量离心管中,并储存于 -20°C。避免反复冻融循环,以免损坏IgG和影响产品性能
法律資訊
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類(WGK)
nwg
閃點(°F)
Not applicable
閃點(°C)
Not applicable
Differential expression of microRNA expression in tamoxifen-sensitive MCF-7 versus tamoxifen-resistant LY2 human breast cancer cells.
Cancer letters null
Stem cell reports, 9(1), 108-121 (2017-06-06)
Of the thousands of long noncoding RNAs expressed in embryonic stem cells (ESCs), few have known roles and fewer have been functionally implicated in the regulation of self-renewal and pluripotency, or the reprogramming of somatic cells to the pluripotent state.
Cell death & disease, 10(3), 168-168 (2019-02-20)
Cholestasis induces the hepatic long non-coding RNA H19, which promotes the progression of cholestatic liver fibrosis. However, microRNAs that are dysregulated by H19 during cholestasis remain elusive. Using miRNA-sequencing analysis followed by qPCR validation, we identified marked upregulation of eight
Brain research, 1436, 20-33 (2011-12-27)
In recent years, microRNAs or miRNAs have been proposed to target neuronal mRNAs localized near the synapse, exerting a pivotal role in modulating local protein synthesis, and presumably affecting adaptive mechanisms such as synaptic plasticity. In the present study we
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 51(3), 1103-1118 (2018-11-27)
Cellular senescence, or permanent growth arrest, is known as an effective tumor suppressor mechanism that can be induced by different stressors, such as oncogenes, chemotherapeutics or cytokine cocktails. Previous studies demonstrated that the growth-repressing state of oncogene-induced senescent cells depends
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