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化驗
>99% (TLC)
形狀
liquid
包裝
pkg of 1 × 1 mL (810604C-1mg)
製造商／商標名
Avanti Research™ - A Croda Brand 810604C
濃度
1 mg/mL (810604C-1mg)
脂質類型
ESR probes
運輸包裝
dry ice
儲存溫度
−20°C
一般說明
1-palmitoyl-2-stearoyl-(16-doxyl)-sn-glycero-3-phosphocholine (16:0-16 Doxyl PC) is a spin labelled phospholipid.
Avanti′s nitroxide spin product listing is a group of compounds designed to act as membrane probes. A variety of positions down the hydrophobic chain are labeled with the nitroxide functional groups to allow probing the membrane at various depths. These compounds have been synthesized from 1-palmitoyl-2-hydroxy-sn-glycerol-3-phosphocholine with the product being purified by column chromatography. Various n-doxyl phosphocholines have been recently used as biophysical tools to elucidate membrane trafficking with phosphatidylinositol transfer proteins and as fluorescent quenchers in lipid bilayer structural studies.
應用
1-palmitoyl-2-stearoyl-(16-doxyl)-sn-glycero-3-phosphocholine (16:0-16 Doxyl PC) may be used:
- in bilayers or membranes to study the distribution of the lipid-attached doxyl electron paramagnetic resonance (EPR) spin label
- as spin-labelled lipid in multilamellar vesicles (MLVs) to measure membrane fluidity
- as a spin-labeled deep quencher in small unilamellar vesicles (SUVs) for nitroxide lipid quenching experiments
包裝
5 mL Clear Glass Sealed Ampule (810604C-1mg)
準備報告
Product use: To prevent aggregation, prepare water-based solutions of 2 mM stock solutions of n-DOXYL PCs and store in plastic. Dilute stock solutions to 0.03- 0.1 mM solutions for EPR studies. For liposome preparations in fluorescent quenching measurements, dissolve the doxyl lipid in 150 μl absolute ethanol for a concentration of 40.3 mM , Additional supplemental information.
法律資訊
Avanti Research is a trademark of Avanti Polar Lipids, LLC
訊號詞
Danger
危險分類
Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 - STOT SE 3
標靶器官
Central nervous system, Liver,Kidney
水污染物質分類（WGK）
WGK 3
Anjali Gupta et al.
Journal of lipid research, 61(2), 252-266 (2019-12-21)
A fundamental feature of the eukaryotic cell membrane is the asymmetric arrangement of lipids in its two leaflets. A cell invests significant energy to maintain this asymmetry and uses it to regulate important biological processes, such as apoptosis and vesiculation.
Alexander Vogel et al.
Biophysical journal, 85(3), 1691-1701 (2003-08-29)
The distribution of the lipid-attached doxyl electron paramagnetic resonance (EPR) spin label in 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine membranes has been studied by (1)H and (13)C magic angle spinning nuclear magnetic resonance relaxation measurements. The doxyl spin label was covalently attached to the 5th
An Ghysels et al.
Nature communications, 10(1), 5616-5616 (2019-12-11)
The functional significance of ordered nanodomains (or rafts) in cholesterol rich eukaryotic cell membranes has only begun to be explored. This study exploits the correspondence of cellular rafts and liquid ordered (Lo) phases of three-component lipid bilayers to examine permeability.
Phosphatidylserine dynamics in cellular membranes.
Kay, J.G
Molecular Biology of the Cell, 23, 2198-2212 (2012)
Insight into antibody combining sites using nuclear magnetic resonance and spin label haptens
McConnell HM
Advances in Protein Chemistry, 49, 135-148 (1996)
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