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儲存溫度
−20°C
一般說明
The Dione MicroMap Kit provides a method for small molecule intracellular target identification via photocatalytic proximity labeling. The kit contains a catalyst that can be coupled to an acid on almost any small-molecule ligand. The subsequent ligand-Iridium conjugate can be used to perform multiple target identification experiments. The following kit provides enough Ir-catalyst to synthesize 20 μMoles of iridium–ligand conjugate and enough Diazirine-PEG3-Biotin to perform 10 proteomics experiments.
The kit provides a step-by-step procedure for conjugating your ligand of choice to a photocatalyst that localizes light-mediated crosslinking to the ligands target. The ligand–iridium conjugate can then be incubated with live cells along with a biotinylated diazirine reagent. Analogous to a satellite, the photocatalyst localizes carbene generation from the diazirine only in the direct vicinity and thus covalently modifies the target protein in a catalytic manner, amplifying the signal for downstream analysis.
To link to an amine on a small molecule (instead of an acid), the Hyas Kit is available.
Photoreactors commercially available:
The kit provides a step-by-step procedure for conjugating your ligand of choice to a photocatalyst that localizes light-mediated crosslinking to the ligands target. The ligand–iridium conjugate can then be incubated with live cells along with a biotinylated diazirine reagent. Analogous to a satellite, the photocatalyst localizes carbene generation from the diazirine only in the direct vicinity and thus covalently modifies the target protein in a catalytic manner, amplifying the signal for downstream analysis.
To link to an amine on a small molecule (instead of an acid), the Hyas Kit is available.
Photoreactors commercially available:
- Penn PhD Photoreactor M2 (Z744035)
- Penn PhD Photoreactor M2 365nm Light Source (Z744031)
- LightOX PhotoReact 365 (Z744061)
Read more about: - Target identification with HyasKit and DioneKit in our Technology Spotlight
- Small molecule photocatalysis enables drug target identification via energy transfer
應用
Proximity protein labeling, protein-protein interactions, photoaffinity labeling, proteomics
生化／生理作用
Kit of components packaged in individual vials for enabling photoinduced proximity labeling. The kit contains an Ir-catalyst to couple to an acid on a small molecule ligand. Kit contains Ir-catalyst and Diazirine PEG3 Biotin to perform 10 proteomics experiments. To link to an amine on a small molecule, the Dione Kit is available.
儲存類別代碼
10 - Combustible liquids
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