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Merck

900584

Sigma-Aldrich

5-乙炔基-2′-脱氧尿苷

95%

别名:

EdU

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About This Item

经验公式(希尔记法):
C11H12N2O5
CAS号:
分子量:
252.22
分類程式碼代碼:
12352208
NACRES:
NA.22

品質等級

化驗

95%

形狀

powder

mp

206 °C

儲存溫度

−20°C

SMILES 字串

OC[C@@H]1[C@@H](O)C[C@H](N2C(NC(C(C#C)=C2)=O)=O)O1

InChI

1S/C11H12N2O5/c1-2-6-4-13(11(17)12-10(6)16)9-3-7(15)8(5-14)18-9/h1,4,7-9,14-15H,3,5H2,(H,12,16,17)/t7-,8+,9+/m0/s1

InChI 密鑰

CDEURGJCGCHYFH-DJLDLDEBSA-N

一般說明

5-乙炔基-2′-脱氧尿苷(EdU)是一种胸苷类似物,可掺入到细胞DNA中,以进行细胞增殖研究。所掺入的核苷类似物可以通过与荧光叠氮化物的铜催化点击反应来检测。

應用

5-乙炔基-2′-脱氧尿苷(EdU)用于:
  • 在复制过程中标记新合成的DNA
  • 细胞增殖试验研究
  • 细胞周期分析
有关包含所有检测试剂的Baseclick试剂盒的信息,请参阅:Baseclick试剂盒

有关Baseclick试剂盒的列表,参见:Baseclick试剂盒

象形圖

Health hazard

訊號詞

Danger

危險聲明

危險分類

Muta. 1B - Repr. 2

儲存類別代碼

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Synthesis, susceptibility to enzymatic phosphorylation, cytotoxicity and in vitro antiviral activity of lipophilic pyrimidine nucleoside/carborane conjugates.
Bialek-Pietras M, et al.
Journal of Organometallic Chemistry, 865, 166-172 (2018)
5-Ethynyl-2?-deoxycytidine as a new agent for DNA labeling: detection of proliferating cells
D Qu, et.al.
Analytical Biochemistry, 417, 112-121 (2011)
Click chemistry as a reliable method for the high-density postsynthetic functionalization of alkyne-modified DNA.
Gierlich J, et al.
Organic Letters, 8(17), 3639-3642 (2006)
Site-directed spin-labeling of nucleic acids by click chemistry: detection of abasic sites in duplex DNA by EPR spectroscopy.
Jakobsen A, et al.
Journal of the American Chemical Society, 132(30), 10424-10428 (2010)
Timothy J Mead et al.
Methods in molecular biology (Clifton, N.J.), 1130, 233-243 (2014-02-01)
Assessing cell proliferation in situ is an important phenotyping component of skeletal tissues from development to adult stages and disease. Various methods exist including immunostaining for proteins and protein modifications associated with specific steps of the cell cycle, but the

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