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一般說明
TransPlex®，一种全转录组扩增（WTA）方法，能够在4小时内，代表性地扩增出纳克级的总RNA而没有3′-偏差。 从组织、培养细胞、福尔马林固定样品或血清中所获得的微克级扩增产物适用于例如qPCR和微阵列分析等下游应用。
應用
TransPlex®全转录组扩增试剂盒已用于合成双链cDNA。还可用于cDNA扩增。
适于各种下游应用，包括：
- qPCR
- 微阵列分析
- 克隆
特點和優勢
- 在4小时内对总RNA进行扩增，手动操作时间低于30分钟
- 仅需5 ng起始材料，即可从总RNA扩增为极具代表性的文库
- 以组织、培养的细胞、血清或来自福尔马林固定的石蜡包埋样品的降解RNA为原料，从完整的RNA可生成微克级的扩增产物
原則
WTA过程包括2个步骤。首先，使用由拟随机3′端和通用5′端组成的非自身互补引物对样品RNA进行逆转录。随着聚合作用的发生，被取代的单链将作为引物退火和延伸的新模板。所得OmniPlex cDNA文库由以通用末端序列为侧翼序列、含100-1000个碱基的随机重叠片段组成。随后，使用通用引物对文库进行PCR扩增，生成WTA产物。
法律資訊
TransPlex is a registered trademark of Rubicon Genomics, Inc.
訊號詞
Warning
危險聲明
危險分類
Eye Irrit. 2
儲存類別代碼
10 - Combustible liquids
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Complete genome sequence of avian paramyxovirus type 7 (strain Tennessee) and comparison with other paramyxoviruses
Xiao S, et al.
Virus Research, 145(1), 80-91 (2009)
Sa Xiao et al.
Virus research, 145(1), 80-91 (2009-06-23)
The complete genome sequence of avian paramyxovirus serotype 7 (APMV-7) prototype strain dove/Tennessee/4/75 was determined. The genome size is 15,480 nucleotides (nt) long and follows the "rule of six". The genome contains six non-overlapping genes in the order of 3'-N-P/V/W-M-F-HN-L-5'.
Catarina Guimarães-Teixeira et al.
Journal of personalized medicine, 11(10) (2021-10-24)
(1) Background: Methylation of N6-adenosine (m6A) is the most abundant messenger RNA (mRNA) modification in eukaryotes. We assessed the expression profiles of m6A regulatory proteins in renal cell carcinoma (RCC) and their clinical relevance, namely, as potential biomarkers. (2) Methods:
Munehiro Okamoto et al.
Scientific reports, 5, 8850-8850 (2015-03-07)
We discovered a lethal hemorrhagic syndrome arising from severe thrombocytopenia in Japanese macaques kept at the Primate Research Institute, Kyoto University. Extensive investigation identified that simian retrovirus type 4 (SRV-4) was the causative agent of the disease. SRV-4 had previously
Eszter Posfai et al.
Genes & development, 26(9), 920-932 (2012-04-14)
In mammals, totipotent embryos are formed by fusion of highly differentiated gametes. Acquisition of totipotency concurs with chromatin remodeling of parental genomes, changes in the maternal transcriptome and proteome, and zygotic genome activation (ZGA). The inefficiency of reprogramming somatic nuclei
商品
Epigenetic modifications are thought to occur through two key interconnected processes—DNA methylation and the covalent modification of histones.
实验方案
Amplification products generated by the TransPlex® WTA and Complete WTA2 kits are suitable for microarray target for expression analyses, and can be incorporated into existing Illumina workflows.
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