G5160

β-Galactosidase from Aspergillus oryzae

≥8.0 units/mg solid

• Synonym(s): β-D-Galactoside galactohydrolase, Lactase from Aspergillus oryzae
• CAS Number: 9031-11-2
• Enzyme Commission number: 3.2.1.23 (BRENDA, IUBMB)
• EC Number: 232-864-1
• MDL number: MFCD00130623
• UNSPSC Code: 12352204

Properties

• form: solid
• Quality Level: 200
• specific activity: ≥8.0 units/mg solid
• contains: dextrin as extender
• storage temp.: −20°C

Description

General description

β-Galactosidase is a member of the glycoside hydrolase clan A (GH-A) family. It is widely present in microorganisms, which includes both prokaryotes and eukaryotes.

Application

β-Galactosidase from Aspergillus oryzae has been used:

• in in vitro nuclear magnet resonance (NMR)
• as a standard in the β-galactosidase assay to determine the concentration of cell-associated β-galactosidase
• to modify sugar residues on the surface of the mutant slime cells to study the adhesive properties
• for enzymatic hydrolysis of lactose
• for reversed-phase (RP) adsorption
• in the hydrolysis of whey lactose

Biochem/physiol Actions

β-Galactosidase acts on the terminal β-D-galactosyl moieties of disaccharides, glycoconjugates, and polysaccharides. It is useful in mediating transglycosylation reactions such as the synthesis of galactooligosaccharides (GOS). β-Galactosidases might be used for glycobiological and biotechnological applications.

β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.

Unit Definition

One unit will hydrolyze 1.0 μmole of lactose per minute at pH at 4.5 at 30 °C.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves