G4142
β-Galactosidase from bovine testes
ammonium sulfate suspension, 1.0-3.0 units/mg protein (modified Warburg-Christian)
Synonym(s):
β-D-Galactoside galactohydrolase
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
Recommended Products
biological source
bovine
Quality Level
form
ammonium sulfate suspension
specific activity
1.0-3.0 units/mg protein (modified Warburg-Christian)
foreign activity
α- and β-glucosidase ≤0.2%
α-fucosidase ≤0.2%
α-galactosidase ≤0.2%
α-mannosidase ≤0.2%
β-N-acetylglucosaminidase ≤1.0%
storage temp.
2-8°C
Application
β-Galactosidase from bovine testes was used for coupling to Sepharose.
Biochem/physiol Actions
β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.
Unit Definition
One unit will hydrolyze 1.0 μmole of p-nitrophenyl β-D-galactopyranoside to p-nitrophenol and D-galactopyranose per min at pH 4.4 at 25 °C.
Physical form
Suspension in 3.2 M (NH4)2SO4, pH approx. 5.0
inhibitor
Product No.
Description
Pricing
substrate
Product No.
Description
Pricing
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
The Journal of biological chemistry, 263(19), 9339-9344 (1988-07-05)
Cloning and sequencing of the human type II insulin-like growth factor (IGF) receptor cDNA revealed an 80% deduced amino acid sequence homology with the bovine cation-independent mannose 6-phosphate (Man-6-P) receptor, suggesting identity of the two receptors (Morgan, D. O., Edman
The purification and properties of beta-galactosidase from bovine testes.
The Journal of biological chemistry, 248(19), 6772-6780 (1973-10-10)
Protein science : a publication of the Protein Society, 8(1), 122-136 (1999-04-21)
Beta-galactosidase (lacZ) from Escherichia coli is a 464 kDa homotetramer. Each subunit consists of five domains, the third being an alpha/beta barrel that contains most of the active site residues. A comparison is made between each of the domains and
Screening of high-risk Gaucher disease patients using dried blood spots techniques.
Gene, 523(1), 114-115 (2013-04-10)
JAMA otolaryngology-- head & neck surgery, 139(2), 173-182 (2013-02-23)
To examine the therapeutic effects of an attenuated, replication-competent vaccinia virus (GLV-1h68) against a panel of 5 human salivary gland carcinoma cell lines. The susceptibility of 5 salivary gland carcinoma cell lines to infection and oncolysis by GLV-1h68 was assessed
Articles
Instructions for working with enzymes supplied as ammonium sulfate suspensions
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service