P3738

Protein Kinase G Iβ human

≥95% (SDS-PAGE), recombinant, expressed in baculovirus infected Sf9 cells, buffered aqueous glycerol solution

• Synonym(s): cyclic-Guanosine Monophosphate-dependent Protein Kinase 1β human
• Enzyme Commission number: 2.7.11.12 (BRENDA, IUBMB)
• MDL number: MFCD03458230
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• recombinant: expressed in baculovirus infected Sf9 cells
• Quality Level: 200
• Assay: ≥95% (SDS-PAGE)
• form: buffered aqueous glycerol solution
• specific activity: ≥1.5 units/mg protein (20-fold stimulation by cGMP (5 μM))
• mol wt: 76 kDa (monomer)
• UniProt accession no.: Q13976
• relevant disease(s): cancer
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... PRKG1(5592)

Description

Application

Protein Kinase G is a serine/threonine-specific protein kinase that is activated by cGMP. Protein Kinase G Iβ is used to induce apoptosis and inhibit cell proliferation.

Biochem/physiol Actions

Protein Kinase G Iβ induces apoptosis in certain cell lines such as human breast cancer cell lines MCF-7 and MDA-MB-468. It inhibits cell proliferation and induces apoptosis in colon cancer cell lines.

Unit Definition

One unit will phosphorylate 1 micromole of VASPtide(RRKVSKQE) substrate per minute in 10 mM HEPES, pH 7.4, 5 mM MgCl2, 1 mM DTE and 0.2 mM EDTA.

Physical form

Solution in 20 mM Tris buffer, pH 7.4, 1 mM EDTA, 1 mM β-mercaptoethanol, 100 mM NaCl, 10 U/ml Trasylol, and 50% glycerol.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable