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GE17-3712-03

NI Sepharose Excel

Cytiva 17-3712-03, pack of 500 mL

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About This Item

UNSPSC Code:
41106500
NACRES:
NA.56

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

packaging

pack of 500 mL

manufacturer/tradename

Cytiva 17-3712-03

matrix

highly cross-linked 6% agarose

average diameter

90 μm

cleaning in place

2-14(Cleaning-in-place: pH interval where the medium can be subjected to cleaning-in-place without significant change in function.)

working range

2-13

capacity

≥10 mg binding capacity (histidine-tagged protein)(Dynamic binding capacity was tested with 0.5 mg/ml (histidine)6- tagged pure protein (Mr 43 000) in EX-CELL® 420 Insect serum-free medium (capacity at 10% breakthrough) or (histidine)6-tagged protein (Mr 28 000). Column volume was 1 ml and flow rate 1 ml/min. Binding capacity is sample-dependent.)

Related Categories

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany
Sepharose is a trademark of Cytiva

Pictograms

Environment

Signal Word

Warning

Hazard Statements

Storage Class Code

3 - Flammable liquids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

This page shows how to optimize purification of histidine-tagged proteins using GE Healthcare products.

This page describes principles and standard conditions for different purification techniques of histidine-tagged proteins using GE Healthcare products.

This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from GE Healthcare.

This page shows troubleshooting instructions for affinity chromatography of tagged proteins using GE Healthcare products.

Protocols

This page how to remove histidine tags by enzymatic cleavage using GE Healthcare products.

This page shows how to purify histidine-tagged proteins secreted into eukaryotic cell culture supernatants using Ni Sepharose Excel from GE Healthcare.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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