GE17-0575-01
Chelating Sepharose™ Fast Flow
Cytiva 17-0575-01, pack of 50 mL
About This Item
Recommended Products
ligand
iminodiacetic acid
packaging
pack of 50 mL
manufacturer/tradename
Cytiva 17-0575-01
matrix
6% cross-linked agarose
particle size
45-165 μm
average diameter
90 μm
cleaning in place
2-14
working range
3-13
suitability
suitable for bioprocess medium
Related Categories
General description
Chelating Sepharose™ Fast Flow is composed of cross-linked 6% agarose beads modified with iminodiacetic immobilized to the base matrix by stable ether linkages and sufficiently long spacer arms.
Chelating Sepharose™ Fast Flow is supplied free of metal ions, allowing the user to charge it with the most appropriate metal ion for purification of a target protein.
Chelating Sepharose™ Fast Flow is available in a range of different bulk pack sizes for easy scale-up and process development. As member of the BioProcess media range, Chelating Sepharose™ Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support
Features and Benefits
- Uncharged IMAC resin allowing possibility to charge with your metal ion of choice for optimized selectivity
- Convenient purification of native and recombinant proteins
- As BioProcess medium it fulfills industrial demands for security of supply, robust performance and regulatory support.
- BioProcess medium supported for industrial applications and well-established in approved processes
Storage and Stability
Analysis Note
Legal Information
Signal Word
Warning
Hazard Statements
Precautionary Statements
Storage Class Code
3 - Flammable liquids
Certificates of Analysis (COA)
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Articles
This page shows how to separate proteins and peptides with affinity for metal ions by immobilized metal chelate affinity chromatography using HiTrap Chelating HP, Chelating Sepharose Fast Flow,His MicroSpin Purification Module or HisTrap Kit from GE Healthcare.
This page shows how to purify or remove proteins and peptides with exposed amino acids with Chelating Sepharose High Performance, Chelating Sepharose Fast Flow, Capto Chelating from GE Healthcare.
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
Protocols
This page shows how to perform a separation with poly (His) fusion proteins which are affinity chromatography tags used in His MicroSpin Purification Module, HisTrap Kit, HiTrap Chelating HP and Chelating Sepharose Fast Flow products from GE Healthcare.
Column packing and preparation for Affinity Chromatography with specific groups of biomolecules.
This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.
This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from GE Healthcare.
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