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Key Documents

GE17-0090-10

Sephadex® LH-20

Cytiva 17-0090-10, pack of 25 g

Synonym(s):

Sephadex gel

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About This Item

UNSPSC Code:
23151817
NACRES:
NA.56

feature

autoclavable 20 min at 121°C

packaging

pack of 25 g

manufacturer/tradename

Cytiva 17-0090-10

matrix

hydroxypropylated, cross-linked dextran

particle size

163 μm (in methanol max.)
18-111 μm (dry)
27 μm (In Methanol Min.)

cleaning in place

2-13

working range

2-13

General description

Sephadex® LH20 is a liquid chromatography media for molecular sizing of natural product such as steroid, terpenoid, lipid, low molecular weight peptide. Useful to analytical and industrial scale for preparation of closely related molecular species.

Application

Sephadex® LH-20 is prepared by hydroxypropylation of Sephadex® G-25, a bead-formed Dextran medium, and has been specifically developed for gel filtration of natural products, such as steroids, terpenoids, lipids and Low molecular weight peptides, in organic solvents.

Features and Benefits

  • Designed for use in polar organic solvents and aqueous solvent mixtures
  • Excellent for fractionation of lipids, hormones, vitamins, and other small biomolecules.
  • Useful for both analytical and industrial scales for the preparation of closely related molecular species
  • Sephadex® LH-20 is made from hydroxypropylated Dextran beads that have been crosslinked to yield a polysaccharide network.
  • Powerful chromatographic selectivity due to dual hydrophilic and lipophilic nature of the matrix
  • Excellent batch-to-batch reproducibility
  • Sephadex® LH-20 is made from hydroxypropylated Dextran beads that have been crosslinked to yield a polysaccharide network.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 25 °C ()

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sephadex is a registered trademark of Cytiva

Storage Class Code

13 - Non Combustible Solids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Protocols

Packing a Column and Performing a Separation with Sephadex® LH-20

This page shows how to perform a separation with a Sephadex LH-20 column from GE Healthcare which are Size Exclusion Chromatography media of polysaccharide network made from cross-linked hydroxypropylated dextran beads.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service