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packaging
pkg of 20 vials (4x50μL aliquots for each of the 5 kit components)
application(s)
CRISPR
shipped in
dry ice
storage temp.
−70°C
General description
Human CRISPRi Unassigned (Novel Targets) Kit contains 2 sub-pools of top 5 gRNAs per gene as well as a separate sub-pool of 5 supplemental gRNAs per gene for increased sensitivity and includes non-targeting controls built in. All pools are functionally titered based on # of guides to provide approximately 3 replicates depending on cell line at 500 gRNAs per cell. Vectors contain both BFP and puromycin as selection markers and an optimized scaffold for sgRNA expression and function. Each sub pool of the library includes non-targeting sgRNA controls. The UCOE KRAB-dCas9 effector is designed for consistent expression and inhibition allowing gene silencing loss-of-function screening with less risk of toxicity to your cells due to double stranded breaks which make the CRISPRi system the ideal choice for perturbation of non-coding genes, applications that call for a reversible, titratable solution, and essential genes.
- Individual CRISPRi clones are easily ordered online or by contacting your local sales representative
- Custom pools for follow up screening or 10x Genomics Compatible CRISPR pools are also available by contacting your local sales representative
For more information about screening with CRISPRi/a please Click Here.
Application
- Functional Genomics/Target Validation
- Focused forward genetic screening
- Set up and optimization of screen assay
- Creation of cell lines stably expressing KRAB-dCas9
- Validated positive and negative controls
Features and Benefits
- Complete ready to use screening kit, including targeted screening pool, effector and positive and negative control
- Best in Class UCSF gRNA selection algorithm and optimized (F+E) gRNA scaffold
- Ease of optimization: Validated positive control targeting RAB1A and non-targeting control for assay set up
- UCOE KRAB-dCas9 for consistent effector expression across a wide variety of cell lines
- Libraries provided at high functional titer, based on FACs or CFU analysis
- Use puromycin and or BFP as selection markers
- Gene targets from UCSF organized into functional categoriy of Unassigned (Novel Targets)
Components
CRISPRIL13: CRISPRi Pool H7 Top5 Unassigned
CRISPRIL14: CRISPRi Pool H7 Supp5 Unassigned
2 Controls and 1 Effector Construct with a minimum concentration of 5x105 TU/mL(via FACS or CFU assay) CRISPRIE: KRAB-dCAS9 CRISPRi Construct Lentiviral Transduction Particles
CRISPRI01: CRISPRi Rab1a Control Lentiviral Transduction Particles
CRISPRI06: CRISPRi Nontargeting Control Puromycin Transduction Particles
Principle
Storage Class Code
12 - Non Combustible Liquids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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