ZRB2211

Anti-Cdc25A Antibody, clone 1M11 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): Dual specificity phosphatase Cdc25A, EC:3.1.3.48, M-phase inducer phosphatase 1
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1M11, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 59.09 kDa; observed mol wt ~54 kDa
• purified by: using Protein A
• species reactivity: human
• packaging: antibody small pack of 25
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• technique(s): affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: C-terminal half
• Protein ID accession no.: NP_001780
• UniProt accession no.: P30304
• greener alternative category: Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: human ... CDC25A(993)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1M11 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Cdc25A. It targets an epitope within 24 amino acids from the C-terminal half.

Immunogen

KLH-conjugated linear peptide corresponding to 24 amino acids from the C-terminal half of human Cdc25A.

Application

Quality Control Testing

Evaluated by Western Blotting in HeLa cell nuclear extract.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Cdc25A in HeLa cell nuclear extract.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Cdc25A in HEK293 cell nuclear extract and 1:10,000 dilution in HepG2 cell lysate.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Cdc25A in Human esophagus tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound Cdc25A peptide with a KD of 5.5 x 10-6 in an affinity binding assay.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Cdc25A in A431, HeLa, and HepG2 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

M-phase inducer phosphatase 1 (UniProt: P30304; also known as EC:3.1.3.48, Dual specificity phosphatase Cdc25A) is encoded by the CDC25A gene (Gene ID: 993) in human. The Cdc25 dual phosphatase family has three members: Cdc25A, Cdc25B, and Cdc25C that contain well conserved catalytic domains, but differ in their regulatory domains. Cdc25A is a dual-specificity protein tyrosine phosphatase that plays a crucial role in cell cycle regulation. It removes the inhibitory phosphorylation in various cyclin-dependent kinases (CDKs) and positively regulates CDK activities leading to cell cycle progression. The regulatory domain of Cdc25A is localized in the N-terminal region and its catalytic domain resides in the C-terminal region. The regulatory domain contains a series of phosphorylation sites that are involved in maintaining its stability and in protein-protein interaction. The N-terminal region also contains the nuclear localization sequence (NLS) and the nuclear export sequence (NES) that determine its subcellular location. Cdc25A plays a role in assisting both G1/S and G2/M progression by dephosphorylating CDK4 at tyrosine 17, CDK6 at tyrosine 24, and CDK2 and CDK1 at threonine 14/tyrosine 15. Its phosphorylation on serine 76, 124, 178, 279, and 293 and threonine 507 by CHEK1 and on serine 124 and 293 by CHEK2 mediates cell cycle arrest. Overexpression of Cdc25A is reported to promote tumorigenesis and is frequently observed in various types of cancer. Two isoforms of Cdc25A have been described that are produced by alternative splicing. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Shen, T., and Huang, S. (2012). Anticancer Agents Med. Chem. 12(6); 631-639; Boutros, R., et al. (2007). Nat. Rev. Cancer. 7(7); 495-507).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable