14-450-M

Cdk1/cyclin B Protein, active, 10 µg

Active, C-terminal His6-tagged human full length Cdk1 & N-terminal GST-tagged human full length Cyclin B, for use in Kinase Assays.

• UNSPSC Code: 12352200
• eCl@ss: 32160405
• NACRES: NA.26

Properties

• biological source: human
• Quality Level: 100
• mol wt: M_w 35 kDa (cdk1); M_w 75 kDa (cyclin B)
• manufacturer/tradename: Upstate^®
• technique(s): activity assay: suitable (kinase)
• NCBI accession no.: NM_001786.2; NM_033379.2
• UniProt accession no.: P06493

Description

General description

C-terminal His6-tagged human full length cdk1, and N-terminal GST-tagged human full length cyclin B

Product Source: expressed individually by baculovirus in Sf21 insect cells

Biochem/physiol Actions

Protein Target: CDK1/cyclinB

Target Sub-Family: CMGC

Packaging

Also available in 250 µg size --call for pricing and availability and reference catalog number 14-450M when ordering the 250 µg size.

Quality

routinely evaluated by phosphorylation of the Histone H1 substrate

Physical form

Purified using Ni²⁺/NTA-agarose and GST-agarose, respectively. The cdk1 is then activated using CAK and repurified by Q Sepharose^™ and Ni2+/NTA-agarose

Storage and Stability

6 months at -70°C

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

Sepharose is a trademark of Cytiva

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.