Ascentis^® Express HILIC U/HPLC Columns

HILIC for separation of polar compounds

HILIC chromatography is a variation of normal-phase chromatography with the distinction that one of the major components of the mobile phase is water. The high volatility of the mobile phase makes HILIC LC-MS friendly, and allows for increased MS sensitivity. Many classes of polar compounds can be retained on the Ascentis^® Express HILIC column including polar neutrals, polar acids, and polar and non-polar basic amines. The HILIC column provides orthogonal selectivity to the Ascentis^® Express C18, with the elution typically the opposite of that for C18. Non-polar compounds elute early and the highly polar compounds have greater retention and selectivity.

For high speed and high resolution separations on HPLC and UHPLC instruments, the Fused-Core^_® particle technology of the Ascentis^® Express HILIC column means that efficiency is typically 40% higher in comparison to fully porous particulate columns with same particle size.

Ascentis® Express HILIC Specifications
Silica:	Type B (High purity silica)
Particle Platform:	Superficially porous particles (SPP)
Phase Chemistry:	bare silica
Particle Size:	2.0 µm / 2.7 µm / 5.0 µm
Pore Size:	90 Å
Carbon Load:	unbonded
Surface Area:	120 m²/g / 135 m²/g / 90 m²/g
pH Range:	1 - 8
Max Temperature at Low pH:	60 °C
Max Temperature at High pH:	40 °C
Endcapped:	na

Featured Applications:

• Metabolites - A Serious Challenge for LC/MS Separations and Usual Detection Techniques
• Metabolomic Profiling of Neurospora crassa Fungi Using HILIC and Reversed-Phase LC-MS

HILIC Columns with Fused-Core^® Technology for Retention of Polar Compounds

Ascentis^® Express HILIC is a high-speed, high-performance liquid chromatography column based on a superficially porous Fused-Core^® particle design. The Fused-Core^® particle provides a thin porous shell of high-purity silica surrounding a solid silica core. This particle design exhibits very high column efficiency due to the shallow diffusion paths. Three different particle sizes are available for your specific needs:

2.0 µm particles

Best Fused-Core UHPLC Column

An optimized solution for high-throughput small molecule analysis

2.7 µm particles

Fast HPLC on ANY System

A practical solution that delivers UHPLC performance from any HPLC

5.0 µm particles

The Lab Work-Horse Column

True plug and play solution for improving existing 3 or 5 µm fully porous particle HPLC columns