BIOshell™ Glycan U/HPLC Columns

High resolution U/HPLC separation of Glycans, glycopeptides, polar peptides and isobaric oligosaccharides

The BIOshell™ Glycan column is a high-speed, high-performance liquid chromatography column based on a Fused-Core^® particle design.

The BIOshell™ Glycan stationary phase is a highly polar ligand that possesses five hydroxyl groups tethered to the silica via novel, proprietary chemical linkage. This unique column chemistry is suitable for analysis of oligosaccharides, particularly for protein-linked glycans using the typical mobile phases for hydrophilic interactive liquid chromatography (HILIC) of oligosaccharides.

BIOshell™ Glycan Specifications
Silica:	Type B (High purity silica)
Particle Platform:	Superficially porous particles (SPP)
Phase Chemistry:	Penta-hydroxy
Particle Size:	2.7 µM
Pore Size:	90 Å
Carbon Load:	3.2%
Surface Area:	135 m²/g
pH Range:	2 - 9
Max Temperature at Low pH:	65 °C
Max Temperature at High pH:	40 °C
Endcapped:	No

Featured Application

• HPLC Analysis of Procainamide-Labeled Human IgG Glycans on BIOshell™ Glycan using HILIC-FLR
• HPLC Analysis of Procainamide-Labeled Cetuximab Glycans on BIOshell™ Glycan using HILIC-FLR
• HPLC Analysis of N-Linked Glycans on BIOshell™ Glycan
• HPLC Analysis of a Procainamide-Labeled Dextran Ladder on BIOshell™ Glycan using HILIC-FLR

The Fused-Core^® particle of BIOshell™ Glycan provides a thin porous shell of high-purity silica surrounding a solid silica core. This particle design exhibits very high column efficiency due to the shallow diffusion paths in the 0.5 micron thick porous shell and the small overall particle size of 2.7 microns.

Outstanding Performance in U/HPLC 2.7 µM SPP particle with 90 Å pores. Ideal for the separation of glycans.