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Przygotowanie próbki	Próbka wyrównana przed ekstrakcją przez 7 minut w temperaturze 40 °C, z mieszaniem przy 250 rpm
Próbka/matryca	4 mL rozcieńczonej próbki piwa o niskiej zawartości alkoholu w fiolce z przestrzenią nadpowierzchniową 10 mL
Proces desorpcji	250 °C, 3 min, split 10:1
Ekstrakcja	.Headspace w 40C przez 2 min, mieszanie przy 250 rpm
Włókno SPME	Pk/3 SPME Assy, 100 µm, PDMS FS 24 ga Auto (Red) (57301)
Kolumna	SUPELCOWAX 10, 30 m x 0.25 mm I.D, 0.25 µm (24079)
Piekarnik	40 °C (5 min), 8 °C/min do 70 °C, 20 °C/min do 250 °C, utrzymywane przez 5 min
Gaz nośny	Hel, stały przepływ 1 mL/min
Injection	Split, 10:1
Liner	0.75 mm I.D. SPME
Inj. Temp.	250 °C
.Detektor	MSD
Interfejs MSD	250 °C
Zakres skanowania	m/z 25-300

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Uwaga dotycząca analizy	Tradycyjne metody oznaczania alkoholu w napojach obejmują ciężar właściwy i chromatografię gazową (GC). Ciężar właściwy może nie być w stanie dokładnie zmierzyć poziomów w napojach o niskiej zawartości alkoholu, poniżej 1%. GC jest lepszym podejściem, ponieważ nie ma tego ograniczenia. Jednak bezpośrednie wstrzyknięcie próbek zawierających cukry lub inne nielotne składniki matrycy może spowodować zanieczyszczenie instrumentu GC. Tę wadę GC można przezwyciężyć, próbkując przestrzeń nad próbką za pomocą mikroekstrakcji do fazy stałej (SPME), Przedstawiona tutaj metoda została opracowana specjalnie do oznaczania etanolu w próbkach o niskiej zawartości alkoholu i została wykorzystana do analizy materiału referencyjnego piwa o niskiej zawartości alkoholu. Próbka miała certyfikowaną wartość 0,505% alkoholu objętościowo (% ABV). Przy użyciu SPME w przestrzeni nadpowierzchniowej, średnia zmierzona wartość wyniosła 0,495% ABV, co stanowi 97% dokładności.
Featured Industry	Food and Beverages
Informacje prawne	SUPELCOWAX jest zastrzeżonym znakiem towarowym firmy Sigma-Aldrich Co. LLC
Inne uwagi	SPME Obróbka wstępna próbki: Rozcieńczyć 400 uL próbki do 4 mL za pomocą 0.1M buforem fosforanu sodu (pH 7) zawierającym 25% NaCl
Przydatność	Zastosowanie do SPME, zastosowanie do GC/MS

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Analiza GC/MS etanolu w piwie o niskiej zawartości alkoholu na SUPELCOWAX® 10 po SPME w przestrzeni nadpowierzchniowej z użyciem włókna PDMS 100 µm

Warunki

Opis

Analiza GC/MS etanolu w piwie o niskiej zawartości alkoholu na SUPELCOWAX^® 10 po SPME w przestrzeni nadpowierzchniowej z użyciem włókna PDMS 100 µm