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Merck

Massively parallel single-nucleus RNA-seq with DroNc-seq.

Nature methods (2017-08-29)
Naomi Habib, Inbal Avraham-Davidi, Anindita Basu, Tyler Burks, Karthik Shekhar, Matan Hofree, Sourav R Choudhury, François Aguet, Ellen Gelfand, Kristin Ardlie, David A Weitz, Orit Rozenblatt-Rosen, Feng Zhang, Aviv Regev
ABSTRAKT

Single-nucleus RNA sequencing (sNuc-seq) profiles RNA from tissues that are preserved or cannot be dissociated, but it does not provide high throughput. Here, we develop DroNc-seq: massively parallel sNuc-seq with droplet technology. We profile 39,111 nuclei from mouse and human archived brain samples to demonstrate sensitive, efficient, and unbiased classification of cell types, paving the way for systematic charting of cell atlases.

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Sigma-Aldrich
Nuclei Isolation Kit: Nuclei EZ Prep, sufficient for 25 nuclei preparations (~1-10×107 cells/preparation)
Sigma-Aldrich
KIMBLE Dounce tissue grinder set, 2 mL complete